乙酰水杨酸铜对HCT-116细胞生长的抑制及诱导凋亡作用  

Effect of Cu(Ⅱ)_2(acetylsalicylate)_4 on inhibition of growth and induced apoptosis in HCT-116 human colon adenocarcinoma cells

作　　者：张峰[1,2] 雷秀玲[2] 翟爱峰[2] 刘伟平 

机构地区：[1]泰山医学院药理教研室 [2]昆明医学院药理教研室,云南昆明650031 [3]昆明重金属研究所

出　　处：《泰山医学院学报》2006年第3期189-192,共4页Journal of Taishan Medical College

摘　　要：目的研究乙酰水杨酸铜对HTC—116细胞生长的抑制及诱导凋亡作用,探讨其抗肿瘤作用的机制。方法采用MTT法检测乙酰水杨酸铜对HTC-116细胞的毒性作用；荧光显微镜照相及流式细胞术检测乙酰水杨酸铜诱导HTC-116细胞凋亡的作用。结果乙酰水杨酸铜处理HTC-116细胞48h、72h的IC50值分别为6．10μmol/L和4．48μmol/L；10μmol/L乙酰水杨酸铜处理HTC-116细胞24h镜下可见核分裂及凋亡小体形成,处理48h流式细胞术可检测出明显的亚二倍体峰,凋亡率达34．1%。细胞凋亡发生呈剂量和时间依赖性。结论乙酰水杨酸铜可显著抑制HTC-116细胞生长并诱导其凋亡。Objective： To study the effect of Cu（ Ⅱ ）2 （acetylsalicylate）4 on the inhibition of growth and the induced apeptosis in HCT-116 cells. Methods： The toxicity of Cu （ Ⅱ ）2 （acetylsalicylate） 4 to HCT-116 cells was detected by MTT assay, and the effect on the induced apoptosis was detected by Fluoroscope and flow cytometer. Results： When HCT-116 ceils were treated by Cu （ Ⅱ ） 2 （ acetyl-salicylate ） 4 for 48h and 72h, the IC50 were 6. 10 μmol/L and 4. 48 μmol/L respectively. Nucleus cleavage and apoptotic bodies were observed when HCT-116 ceils were treated by Cu（ Ⅱ ） 2 （acetylsalicylate）4 （10 μmol/L） for 24 h; and an obvious subdiploid peak exhibited when HCT-116 cells were treated by Cu（ Ⅱ ）2 （acetylsalicylate）4 （10 μmol/L） for 48 h, and the rate of apoptosis was 34.1%. Apoptosis was dependent on concentration and time. Conclusion ： The results suggest that Cu （ Ⅱ ） 2 （ acetylsalicylate ） 4 can significantly inhibit the growth of HCT- 116 cells and induce the apoptosis.

关 键 词：乙酰水杨酸铜 人结肠癌细胞 细胞凋亡 

分 类 号：R730.5[医药卫生—肿瘤]