糖尿病大鼠心肌磷酸受纳蛋白基因表达和肌浆网Ca^(2+)-ATPase活性的变化  

Alterations of phospholamban expression and cardiac sarcoplasmic reticulum Ca ～(2+)-ATPase activity in diabetic rats

作　　者：赵晓燕[1] 胡申江[1] 李江[1] 牟云[1] 陈宝平[2] 许蓓[1] 

机构地区：[1]浙江大学医学院附属第一医院心血管内科,浙江杭州310003 [2]浙江大学医学院生理教研室,浙江杭州310031

出　　处：《中国病理生理杂志》2006年第9期1689-1692,共4页Chinese Journal of Pathophysiology

基　　金：浙江省科技厅资助项目(No.021107817);浙江省卫生厅资助项目(No.2003A041);国家教育部留学归国人员科研基金资助(No.1999-747)

摘　　要：目的:观察糖尿病(DM)大鼠心肌磷酸受纳蛋白(PLB)基因表达和肌浆网Ca2+-ATPase活性的变化及其与心功能的关系。方法:复制糖尿病大鼠模型,分别于4、6、8周后对糖尿病组和对照组进行左心室血流动力学检测,测定心肌PLBmRNA转录水平以及蛋白表达水平变化,检测心肌肌浆网Ca2+-ATPase活性。结果:糖尿病大鼠心肌PLBmRNA转录和蛋白表达水平4周时与正常大鼠无明显差异,6周时和8周时明显高于正常大鼠;肌浆网Ca2+-ATPase活性4周时无明显改变,6周时和8周时明显低于正常大鼠;糖尿病大鼠4周时LVSP、LVEDP、±dp/dtmax与正常大鼠无明显差异,6周、8周时LVSP、±dp/dtmax显著降低,LVEDP显著升高。结论:糖尿病大鼠心肌PLB表达水平升高,肌浆网Ca2+-ATPase活性降低,引起心功能下降。AIM： To investigate the alterations of phospholamban （PLB） expression and cardiac sarcoplasmic reticulum （SR） Ca^2＋-ATPase activity, and the change of cardiac function in rats with diabetes mellitus （DM）. METHODS ： The diabetes mellitus in male Wistar rats was induced by intraperitoneal injection of streptozotocin. The levels of PLB mRNA and PLB protein, the activity of SR Ca^2＋-ATPase and the left ventricular hemodynamics parameters were measured 4 weeks, 6 weeks and 8 weeks after DM was induced in rats, while the normal rats served as control group. RESULTS： There was no significant difference in PLB mRNA level and protein level between 4 - week - DM rats and normal control rats. 6 - week - DM rats and 8 - week - DM rats had markedly increased PLB mRNA and protein level compared with normal control rats. SR Ca^2＋-ATPase activity was not significantly changed in 4 - week - DM rats compared with normal control rats, and was markedly depressed in 6 -week- DM rats and 8 -week -DM rats. LVSP, LVEDP and ± dp/dtmax were not significantly changed in 4 - week - DM rats compared with normal control rats. In 6 - week - DM rats and 8 - week - DM rats, LVSP and ± dp/dtmax were decreased, LVEDP was increased compared with normal control rats. CONCLUSION： The elevated levels of PLB mRNA and PLB protein contribute to SR Ca^2＋- ATPase activity reduction, which leads to cardiac dysfunction in DM rats.

关 键 词：糖尿病 磷酸受纳蛋白 肌浆网 CA^2+-ATP酶 基因表达 

分 类 号：R363[医药卫生—病理学]