粒细胞集落刺激因子对大鼠颈动脉球囊损伤后再内皮化和内膜增生过程的影响  

Effect of granulocyte-colony stimulating factor on the reendothelialization and intima hyperplasia of ballon-injured rat carotid artery

作　　者：王晓军[1] 崔连群[2] 刘继东[1] 王敏[3] 盖玉生[1] 李峰[1] 

机构地区：[1]山东大学山东省立医院 [2]山东省立医院内科,山东济南250021 [3]山东科技大学生物医学工程系,山东济南250021

出　　处：《中国病理生理杂志》2006年第9期1702-1707,共6页Chinese Journal of Pathophysiology

摘　　要：目的:探讨重组人粒细胞集落刺激因子对大鼠颈总动脉球囊损伤后再内皮化和内膜增生过程的影响。方法:56只Wistar大鼠随机分为2组,重组人粒细胞集落刺激因子(商品名:瑞白)+损伤组(rhG-CSF预防组)(n=28):皮下注射瑞白30μg/kg,每日1次,共7d后,行左颈总动脉球囊拉伤,术后1h(n=4)、3d(n=4)、5d(n=4)、7d(n=5)、14d(n=6)取材;生理盐水+损伤组(NS+损伤组)(n=28):皮下注射生理盐水0·5mL,同上时点取材。应用扫描电镜、伊文氏蓝染色、HE染色、免疫组织化学的方法,观察颈总动脉损伤后再内皮化、新生内膜增厚及细胞增殖的情况。通过RT-PCR检测血管壁内皮型一氧化氮合酶(eNOS)mRNA表达。结果:重组人粒细胞集落刺激因子可明显增加损伤血管内皮的修复面积、抑制新生内膜形成,增加了血管壁eNOSmRNA表达。结论:重组人粒细胞集落刺激因子对大鼠颈总动脉球囊损伤血管再内皮化和内膜增生过程有血管重塑作用。AIM： To study the effect of mobilization of stem cells by exogenous recombinant human granulocyte - colony stimulating factor （ rhG - CSF） on the repairing process of reendothelialization and neointima hyperplasy on ballon injured rat carotid arteries. METHODS： Male Wistar rats were randomly divided into rhG - CSF group and NS ＋ injury group. The animals were injected daily with 30 μg/kg rhG - CSF or 0. 9% NaCl for 7 days, then underwent balloon angioplasty of the common carotid arteries which were harvested and processed for scanning electron microscopy （ SEM ） , Evans blue staining, morphometric analysis of endothelialization and neointimal formation at 1 h, 3 d, 5 d, 7 d, 14 d after injury. Immunohistochemistry for proliferation cell nuclear antigen （PCNA） and RT- PCR for eNOS mRNA were also conducted for evaluating the proliferation of cells of the vessel wall and the possible mechanism of the repairing. RESULTS： SEM and Evan＇ s blue staining showed increased reendothelialization of the denuded vessels in rhG - CSF - treated animals compared with that NS ＋ injury animals [ （60. 6 ± 7.3 ） % vs （41.6 ± 3.3 ）%, P 〈 0. 01 ]. Neointima thickness was reduced by 37.3% in rhG - CSF group compared with NS ＋ injury group 2 weeks after injury. Immunohistochemical staining for PCNA positive cells was less in rhG - CSF group compared with that in NS ＋ injury group （42.6% vs 72.8%, P 〈 0. 01 ）. CONCLUSION： rhG -CSF has beneficial effects on the reendothelialization and neointima thickness of the ballon -injured arteries. Mobilization of EPCs by exogenous granulocyte colony stimulating factor may be a potential therapeutic strategy for prevention of restenosis after percutenous coronary artery intervention.

关 键 词：粒细胞集落刺激因子 内皮祖细胞 颈动脉 大鼠 

分 类 号：R363[医药卫生—病理学]