胃癌中RUNX3、cyclinE和P21蛋白表达与患者生存的关系  

Relationship between RUNX3,cyclin E,P21 and survival in gastric cancer patients

作　　者：陈金明[1] 干建新[1] 喻春钊[2] 王沈华[1] 伍峻松[1] 易建华[1] 杨检新[1] 周海波[1] 

机构地区：[1]浙江大学医学院附属第二医院外科,浙江杭州310009 [2]江苏省人民医院外科,江苏南京210029

出　　处：《中国病理生理杂志》2006年第9期1766-1770,共5页Chinese Journal of Pathophysiology

摘　　要：目的:探讨胃癌RUNX3蛋白表达对细胞周期蛋白的影响及其与胃癌患者生存和生物学特性的关系。方法:应用免疫组化,分析RUNX3蛋白的表达;应用流式细胞术,分析cyclinE和P21蛋白表达;采用Kaplan-Meier限乘法计算生存率。结果:在56例胃癌中RUNX3蛋白、cyclinE和P21蛋白的阳性表达率分别为44.6%、64.3%和32.1%。胃癌细胞RUNX3蛋白表达与淋巴转移和远处转移有关(P<0.05)。胃癌细胞cyclinE的表达与浸润深度、淋巴转移和远处转移有关(P<0.05)。而胃癌细胞P21的表达与远处转移有关(P<0.05)。胃癌细胞中RUNX3和P21的表达之间呈明显正相关(r=0.57,P<0.05),而与cyclinE的表达之间无相关性(r=0.25,P>0.05)。用Kaplan-Meier生存曲线经Log-rank检验发现,RUNX3和cyclinE的阳性表达与生存相关(P<0.05),P21的阳性表达与生存无关(P>0.05)。结论:RUNX3蛋白可能通过影响P21蛋白的表达影响胃癌的发生发展;检测RUNX3和cyclinE的表达可作为反映胃癌临床病理学特点和预后的指标。AIM： To evaluate the relationship between RUNX3, cyelin E, P21, biological features and survival in gastric cancer patients. METHODS： RUNX3 was examined using immunohistochemical staining. Cyclin E and P21 were analyzed by flow cytometry. Survival was evaluated by Kaplan - Meier survival curves. RESULTS ： The positive - expression rate of RUNX3, cyclin E and P21 in tumor tissue from 56 patients with gastric cancer were 44. 6%, 64. 3% and 32.1% , respectively. RUNX3 expression was correlated with lymph node metastasis and distant metastasis （P 〈 0. 05 ）. Cyclin E might be related to depth of invasion, lymph node metastasis and distant metastasis （P 〈 0. 05 ）. 1921 was correlated with lymph node metastasis （ P 〈 0. 05 ）. It was revealed that RUNX3 and P21 were correlated （ r = 0. 57, P 〈 0. 05 ）, no correlation between RUNX3 and cyclin E was observed （r = 0. 25, P 〉 0. 05 ）. Using Kaplan - Meier survival curves and the Log - rank test, there was correlation between RUNX3, cyclin E and survival （P 〈 0. 05 ）. No correlation between P21 and survival was observed （P 〉 0. 05 ）. CONCLUSION： RUNX3 may be related with tumorigenesis and tumor progression by affecting P21 expression. The detection of RUNX3 and cyclin E may be helpful in evaluating the clinicopathological parameters and prognosis in gastric carcinoma patients.

关 键 词：胃肿瘤 基因 RUNX3 蛋白质P21 幸存 细胞周期蛋白E 

分 类 号：R73[医药卫生—肿瘤]