实验性视网膜脱离时神经生长因子对视网膜细胞凋亡的干预作用  

Interference effect of nerve growth factor on apoptosis of retinal cells in experimental retinal detachment

作　　者：孙晓东[1] 张皙[1] 许迅[1] 余彰[2] 陈荣家[3] 路长林[4] 

机构地区：[1]上海交通大学附属第一人民医院眼科,200080 [2]复旦大学医学院组织形态学电镜教研室 [3]复旦大学附属眼耳鼻喉科医院病理科 [4]上海第二军医大学神经生物教研室

出　　处：《中华眼底病杂志》2006年第5期333-335,共3页Chinese Journal of Ocular Fundus Diseases

摘　　要：目的研究神经生长因子(NGF)在实验性视网膜脱离(RD)时对视网膜细胞凋亡的干预作用。方法27只大鼠左、右眼分别被分为实验对照组和NGF组。视网膜下注射透明质酸钠建立RD动物模型后,取右眼在玻璃体腔内注射1μg/μlNGF,共5μl,作为NGF组;左眼注射磷酸盐缓冲液(PBS)5μl,作为实验对照组。注射方法为每4天注射1次,直至观察期结束。两组在建立模型后1.5、3、6、12h、1、2、4、8、16、32d分别取眼球。另设立正常对照组2只大鼠,不作任何处理,在实验观察结束时取眼球。采用原位末端标记法(TUNEL)和透射电子显微镜观察视网膜细胞凋亡情况,并进行细胞计数和统计学检验。结果RD早期就有细胞凋亡的发生。视网膜各层细胞均可见凋亡细胞,内、外核层分布最多。凋亡细胞数随脱离时间延长而增加(P<0.01)。NGF组的凋亡细胞数比实验对照组少(P<0.01)。结论实验性RD中,玻璃体腔内给予外源性NGF能抑制视网膜细胞凋亡的发生。Objective To investigate the interference effect of nerve growth factor （NGF） on apoptosis of retinal cells in experimental retinal detachment （RD）. Methods Twenty seven Sprague-Dawely rats were selected, and the left and right eyes were in the experimental control group and NGF group, respectively. After the RD model was set up by subretinal injection with sodium hyaluronate, 5μl NGF （1 1μg/μl） was injected into the vitreous body of the right eyes which were in the NGF group; 5μl PBS was injected into vitreous body of left eyes which were in the experimental control group. The injection was performed once every 4 days till the end of the observation period. The eye balls of the 27 rats were extrafted 1. 5, 3, 6, 12 hours, 1 day, 2, 4, 8, 16, and 32 days after the RD model was established. Another 2 rats were selected as the normal control, which underwent none of the injections but eyeball extraction at the end of the observation period. TUNEL and transmission electron microscopy were used to detect the apoptosis of the retinal cells. Cell counts and statistical analysis were used to assess results. Results Typical apoptosis cells were observed in the early time of RD. Apoptosis was found in each retinal layers, especially in inner and outer nuclear layers. The number of apoptosis cells increased as the time of RDwas prolonged（P〈0.01）. It was also found that apoptosis cells in NGFgroup were less than that in the experimental control group（P〈0. 01）. Conclusion Intravitreous injection exogenous NGF may inhibit the apoptosis of retinal cells in experimental RD.

关 键 词：视网膜脱离 神经生长因子 细胞死亡 疾病模型 动物 

分 类 号：R774.1[医药卫生—眼科]