CpG-ODN持续刺激对小鼠骨髓树突状细胞成熟的影响  

The impact of long-term stimulation by CpG-ODN on the maturation of dendritic cells in murine bone marrow

作　　者：陈杰[1] 孙卫民[1] 季海峰[1] 田野苹[1] 

机构地区：[1]第二军医大学免疫研究所

出　　处：《中国肿瘤生物治疗杂志》2006年第4期261-265,共5页Chinese Journal of Cancer Biotherapy

基　　金：国家自然科学基金资助项目(30271232)

摘　　要：目的:研究CpG-ODN持续刺激对小鼠骨髓树突状细胞(DC)成熟的影响。方法:小鼠骨髓细胞用GM-CSF培养7d,持续刺激组全程加入CpG-ODN,短期刺激组在培养的最后36h加入CpG-ODN,对照组不加CpG-ODN。流式细胞仪检测细胞表型和细胞摄取抗原的能力,ELISA检测细胞产生的细胞因子,混合淋巴细胞培养检测细胞提呈抗原的能力。结果:用CpG-ODN持续刺激的小鼠骨髓DC表达MHCⅡ、CD86和CD40等分子和分泌IL-12p70的能力并未增加,吞噬FITC-OVA的能力显著升高,刺激同种异基因T细胞和刺激同种同基因T细胞增殖的能力显著低于CpG-ODN短期刺激组。结论:CpG-ODN持续刺激可抑制DC的发育成熟,可能是持续严重感染时免疫功能低下的原因。Objective： To investigate the effect of a long-term CpG-ODN stimulation on the maturation of murine bonemarrow derived dendritic cells （BMDCs）. Methods： Murine bone-marrow cells were cultured in GM-CSF alone or with CpG-ODN for 7 d or for last 36 h （ days 6, 7）. Cell phenotypes and antigens uptake by BMDCs were analyzed by flow cytometry. Cytokines released by BMDCs were detected by ELISA. The antigen presenting capability by BMDCs was evaluated by mixed lymphocyte responses. Results ： Compared to those of the short-term CpG-ODN stimulation group, the expression of MHC Ⅱ, CD86, CD40, and secretion of IL-12（p70） by BMDCs in long-term stimulation group were not increased. The phagocytosis of FITC-OVA by BMDCs in long-term CpG-ODN stimulation group was strengthened, but the activation of allogenic and homogenic lymphocyte cells proliferation was impaired. Conclusion：Long-term CpG-ODN stimulation can suppress the maturation of DCs, which may explain the low adaptive immunity in sepsis patients.

关 键 词：CPG基序 树突状细胞 成熟 

分 类 号：R730[医药卫生—肿瘤]