杏仁核外源性胃动素对大鼠胃运动的调节作用及机制  

Effect of exogenous motilin in amygdaloid nucleus on gastric motility and its underlying mechanism in rats

作　　者：韩晓华[1] 徐珞[2] 陶尚敏[1] 

机构地区：[1]青岛大学医学院生理教研室,山东省青岛市266021 [2]青岛大学医学院病理生理教研室,山东省青岛市266021

出　　处：《世界华人消化杂志》2006年第22期2159-2163,共5页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30470642

摘　　要：目的:观察双侧基底内侧杏仁核(BMA)内注射胃动素对大鼠胃运动的影响,并探讨胃动素作用的神经传导通路化测定等方法探讨胃动素作用的神经传导通路．方法:选用成年♂Wistar大鼠40只,进行买验1:双侧BMA注射胃动素(MT,1μg/侧)或生理盐水(NS,0．5μL/例),通过胃内球囊-压力换能器-二道生理记录仪观察胃内压(IGP)和胃运动频率(GMF)的改变(n=14);实验2:膈下迷走神经切断后重复实验1(n=12);实验3:BMA注射MT或NS后60 min,采用免疫荧光法观察下丘脑室旁核(PVN)c-Fos蛋白表达(n=7);实验4:放射免疫法测定正常大鼠各脑区胃动素含量(n=7)．结果:双侧BMA注射胃动素后,大鼠胃运动明显加强,给药10,15,20,25 min后,IGP变化百分率分别为19．7％±6．5％(P=0．023), 62．9％±4．7％(P<0．01),45．1％±7．9％(P<0．01), 29．3％±10．3％(P=0．029)．GMF在给药后15和20 min明显增加,其变化百分率分别为36．7％±8．5％(P<0．01)和19．5％±6．0％(P=0．015)．双侧BMA注射NS后IGP和GMF均未见明显改变．大鼠行膈下迷走神经切断后重复实验1．可观察到胃动素促进胃运动的效应完全被阻断(P>0．05)．双侧BMA注射MT后,PVN内c-Fos阳性细胞数较NS对照组明显增多(53．4±8．9 vs 22．5±5．2,P<0.01),c-Fos蛋白表达增强．正常大鼠下丘脑胃动素含量较高(74．3±19．6 mg／kg),其他脑区内胃动素含量为7．8±2．2→17．3±6．6 mg／kg．结论:基底内侧杏仁核外源性胃动素可加强大鼠胃运动,该效应可能通过杏仁核-下丘脑和脑干-迷走神经通路来完成．AIM： To investigate the effect of motilin microinjection into bilateral basomedial amygdaloid nucleus （BMA） on the gastric motility in rats. and explore its underlying mechanism.METHODS： Forty male Wistar rats were used for four sets of experiments （Exp）. Exp 1： Animals received bilateral injections of motilin （MT. 1 μg/side） or normal saline （NS. 0.5 μL/side） into BMA. and both intragastric pressure （IGP） and gastric motility frequency （GMF） were recorded to estimate the gastric motility （n = 14）. Exp 2： Pretreatment of vagotomy was carried out in 12 rats. and then MT or NS was injected into BMA as in Expl. Exp3： Sixty minutes after MT or NS injection into BMA. c-Fos protein expression was detected in the paraventricular nucleus （PVA. n = 7）. Exp 4： Motilin contents were measured in five brain areas （hypothalamus. midbrain. pons. medulla. and pituitary gland）. using radioimmunoassay （RIA） method （n = 7）.RESULTS： Exogenous motilin in BMA enhanced the gastric motility in rats. and this action lasted about 15 min. At the 10th. 15th. 20th. 25th rain after motilin injection. the changes of IGP percentage were 19.7% ± 6.5%（P = 0.023）. 62.9% ± 4.7% （P 〈 0.01）. 45.1% ± 7.9% （P 〈 0.01）. 29.3% ± 10.3% （P = 0.029）. The changes of GMF percentage at the 15th. 20th rain were 36.7% ± 8.5% （P 〈 0.01） and 19.5% ± 6.0% （P = 0.015）. respectively. No obvious changes in both IGP and GMF were observed in normal saline controls. Pretreatment of subdiaphragrnal vagotomy abolished the enhanced gastric motility induced by motilin injection. Motilin injection into bilateral BMA induced increased numbers of c-Fos positive cells in PVN. as compared with the saline control （53.4 ± 8.9 vs 22.5 ± 5.2. P 〈 0.01）. Among the five tested brain areas. the highest motilin level was found in the hypothalamus area （74.3 ± 19.6 mg/kg）. The motilin contents in the other four areas ranged from 7.8 ± 2.2 mg/kg to 17.3 ± 6.6 mg/kg.CONCLUSION： Ex

关 键 词：胃动素 杏仁核 胃运动 迷走神经切断 C-FOS 大鼠 

分 类 号：R-332[医药卫生]