乳糖酶在SD大鼠小肠中的分布情况的研究  

Study on the Distribution of Lactase in the Small Intestine of SD Rats

作　　者：何伟[1] 吕斌[1] 黄承钰[2] 

机构地区：[1]河南省医学科学研究所营养食品研究室,郑州450052 [2]四川大学华西公共卫生学院营养与食品卫生学教研室

出　　处：《四川大学学报（医学版）》2006年第5期785-786,800,共3页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号30271126)资助

摘　　要：目的 研究乳糖酶活性及其mRNA在SD大鼠小肠中的分布特点。方法 3～4周龄SD大鼠15只，自十二指肠下连续剪取三段，每段约10cm，分别测定乳糖酶活性及乳糖酶mRNA水平。结果 从十二指肠起，小、肠乳糖酶活性逐段降低（上、中、下三段分别为0．179、0．160、0．151U／mL匀浆，三组间两两比较．P〈0．05）；三组间乳糖酶mRNA水平差异无统计学意义。从细胞图谱看，SD大鼠乳糖酶mRNA除主要沿肠线分布外，肠绒毛上也有不少散在的分布。结论 乳糖酶mRNA在各个肠段都有分布，但其活性部分主要分布在小肠上段。Objective The article deals with the study on the distribution of lactase and its mRNA in the SD rat small intestine. Methods 15 of the 3-4 wk old SD rats were selected for this research project. From SD rat duodenum, we scissored the small intestine into 3 segments of each 10cm length to determine the lactase activities and mRNA levels. Results The lactase activities of all 3 part intestines were declined from the duodenum stage by stage （the upper, middle and lower segment was 0. 179, 0. 160, or 0. 151 U/ml homogenate respectively, P〈0. 05）, but there were no significant difference of mRNA levels happening among the 3 groups. Conclusion The results suggest that the lactase activity mainly distribute over the upper intestine, although there were lactase along whole intestine.

关 键 词：SD大鼠 乳糖酶 小肠 

分 类 号：Q954.6[生物学—动物学]