透明质酸酶及基质金属蛋白酶-9在人乳腺癌侵袭与转移中的意义  

Expression of hyaluronidase and matrix metaloproteinase-9 in the invasion and metastasis of human breast cancer

作　　者：汪毅[1] 任国胜[2] 石远[2] 王小毅[2] 冉亮[2] 

机构地区：[1]中国医学科学院中国协和医科大学肿瘤医院腹外科,在读博士北京100021 [2]重庆医科大学附属第一医院普外科,重庆400016

出　　处：《中国微创外科杂志》2006年第9期675-677,共3页Chinese Journal of Minimally Invasive Surgery

基　　金：国家自然科学基金资助项目(编号:30070292)

摘　　要：目的研究透明质酸酶(HAase)与基质金属蛋白酶-9(MMP-9)在人乳腺癌侵袭与转移中的意义。方法应用组织匀浆、酶连吸附测定法及免疫组化等方法测定HAase和MMP-9在人乳腺癌中的表达和其病理特征比较。结果①乳腺癌组织学分级Ⅰ、Ⅱ、Ⅲ级组HAase的分别为4.89±2.55、8.03±2.66、12.00±3.96 mU/g(n=10,20,3),Ⅲ级组>Ⅱ级组>Ⅰ级组(P<0.05)。乳腺癌有腋淋巴结转移组HAase表达(8.27±3.26 mU/g,n=23)明显高于无腋淋巴结转移组(5.53±2.82 mU/g,n=10)(t=-2.305,P=0.028)。②MMP-9阳性率在淋巴结转移组为(78.3%,18/23),明显高于无淋巴结转移组(30.0%,3/10)(P=0.016)。③MMP-9阳性组(n=21)HAase(8.57±3.12)mU/g明显高于阴性组(n=12)(5.47±2.85)mU/g(t=2.830,P=0.008)。结论HAase和MMP-9一样,与人乳腺癌侵袭与转移有关。Objective To study the expression of hyaluronidase （HAase） and matrix metalloproteinase-9 （MMP-9 in the invasion and metastasis of human breast cancer. Methods The expression of HAase and MMP-9 in human breast cancer was detected using tissue homogenate preparing, enzyme-linked immunoadsorbent assay （ELSA）, and immunohistochemistry examination. Results ① The levels of HAase were 4.89±2.55, 8.03±2.66, and 12.00±3.96 mU/g in grade Ⅰ, Ⅱ, and Ⅲ of breast cancer, respectively （n = 10, 20, 3）, with significant differences between each other （P 〈 0.05）. The HAase levels were significantly elevated in patients with axillary lymph node metastasis （8.27±3.26 mU/g, n = 23） as compared with patients without metastasis （5.53±2.82 mU/g, n = 10） （t = -2.305, P = 0.028）. ② The positive rate of MMT-9 was significantly higher in patients with axillary lymph node metastasis （78.3%, 18/23） than patients without metastasis （30.0%, 3/10） （P = 0.016）. ③ The expression of HAase was significantly greater in MMT-9 positive patients （8.57±3.12 mU/g, n = 21） than in MMT-9 negative patients （5.47±2.85 mU/g, n = 12） （t = 2.830, P = 0.008）. Conclusions Both HAase and MMT-9 are associated with the invasion and metastasis of human breast cancer.

关 键 词：乳腺癌 透明质酸酶 基质金属蛋白酶-9 侵袭与转移 

分 类 号：R737.9[医药卫生—肿瘤] R446.6[医药卫生—临床医学]