肺耐药蛋白在胰腺癌细胞中的表达及意义  

Expression and Functional Significance of Lung Resistance Protein in Pancreatic Adenocarcinoma Cell

作　　者：郭俊超[1] 闫长青[1] 廖泉[1] 赵玉沛[1] 

机构地区：[1]中国医学科学院北京协和医院基本外科,北京100730

出　　处：《中国普外基础与临床杂志》2006年第5期502-505,共4页Chinese Journal of Bases and Clinics In General Surgery

基　　金：国家自然科学基金项目(编号:30371389)

摘　　要：目的探讨肺耐药蛋白(lungresistanceprotein,LRP)在胰腺癌细胞株中的表达及意义。方法采用反转录多聚酶链反应(RT-PCR)和免疫细胞化学方法检测8株胰腺癌细胞株(SW1990、PCT-2、PCT-3、PCT-4、Aspc-1、Capan-1、Mia-PaCa-2及Panc-1)中LRP在基因水平和蛋白水平的表达。结果RT-PCR结果显示,在胰腺癌细胞株PCT-2中未检测到LRPmRNA的表达,在PCT-4、Aspc-1和Panc-1中,LRPmRNA表达条带较强;在SW1990、PCT-3、Capan-1和Mia-PaCa-2中LRPmRNA表达条带较弱;半定量平均表达水平为0.56±0.33。免疫细胞化学结果证实,在PCT-2细胞中无LRP蛋白表达,PCT-3细胞中LRP蛋白弱表达,SW1990、Aspc-1和Capan-1细胞中LRP蛋白中度表达,而在PCT-4、Mia-PaCa-2和Panc-1细胞中可见LRP蛋白的过度表达。结论在胰腺癌细胞中存在LRP的先天性表达,LRP过表达可能是介导胰腺癌细胞先天性耐药的重要机理之一。Objective To detect the expression of lung resistance protein （LRP） and investigate its .significance in pancreatic carcinoma cell lines （SW1990, PCT-2, PCT-3, PCT-4, Aspc-1, Capan-1, Mia-PaCa-2 and Panc-1）. Methods Reverse transcription PCR （RT-PCR） and immunocytochemistry （ICC） were carried out to investigate the expression of LRP. Results LRP mRNA was absent in PCT-2 cell line by RT PCR. Mild to moderate expres- sion level was found in other pancreatic carcinoma cell lines. PCT-4, Aspc-1 and Panc-1 presented the highest LRP mRNA expression level, in contrast, SW1990, PCT-3, Capan-1 and Mia-PaCa 2 showed moderate LRP mRNA ex pression. The median value was 0.56±0.33. LRP was further validated by ICC. Absent to weak protein expression of LRP was found in PCT 2 and PCT 3. Overexpressed LRP was present in SW1990, Capan-1 and Aspc-1, furthermore, the highest expression of LRP was found in Panc-1, Mia-PaCa-2 and PCT-4 cell lines. Conclusion All these data showed that LRP might play an important role in multidrug resistance of pancreatic carcinoma.

关 键 词：胰腺肿瘤 多药耐药 获得性耐药 

分 类 号：R735.9[医药卫生—肿瘤]