环氧合酶-2及血管生成素-2在大肠癌中的表达及临床意义  

Expression and Significance of Cyclooxygenase-2 and Angiopoietin-2 in Colorectal Cancer

作　　者：张宁[1] 陶凯雄[1] 王国斌[1] 田元[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院普外科,武汉430022

出　　处：《中国普外基础与临床杂志》2006年第5期564-567,共4页Chinese Journal of Bases and Clinics In General Surgery

基　　金：湖北省科技攻关计划(编号:2005AA304B09)

摘　　要：目的研究环氧合酶-2(cyclooxygenase-2,Cox-2)及血管生成素-2(angiopoietin-2,Ang-2)在大肠癌组织、癌旁组织及正常大肠组织中的表达及其与大肠癌临床病理特征之间的关系。方法采用免疫组化SP法检测45例大肠癌组织、45例癌旁组织及15例正常大肠组织中Cox-2和Ang-2的表达情况。结果大肠癌组织中的Cox-2和Ang-2的表达阳性率(80.0%,66.7%)均分别高于癌旁组织(35.6%,11.1%)及正常大肠组织(0,0),P均<0.01。在大肠癌组织中Cox-2和Ang-2蛋白表达与淋巴结转移及Dukes分期有关(P<0.05),且二者的表达具有相关性(P<0.01)。结论Cox-2和Ang-2在大肠癌发生、发展中起重要作用,二者在表达上密切相关。Objective To study the expressions of cyclooxygenase-2 （Cox-2） and angiopoietin-2 （Ang-2） in colorectal cancer tissues, cancer adjacent tissues and normal colorectal tissues, and the relationship between these expressions and the clinicopathologic features of colorectal cancer. Methods Forty five excised samples of colorectal adenocarcinoma were confirmed pathologically and 39 of them were of well or moderately differentiated and 6 of poorly differentiated. Lymph nodes metastasis developed in 30 patients. And 15 cases were in stage of A or B and the rest were in the stage of C or D according to the Dukes stage. Taken PBS as the negative control and the verified Cox-2 or Ang-2 positive sections as positive controls, this study detected the expressions of Cox-2 and Ang 2 protein in 45 eolorectal cancer tissues, 45 cancer adjacent tissues and 15 normal colorectal tissues by using immunohistochemical SP technique method. Results Cox-2 and Ang-2 were expressed in colorectal cancer tissues and cancer ad- jacent tissues, but were not expressed in normal colorectal tissues. In 45 colorectal cancer tissues, the positive expression rates of Cox-2 and Ang 2 were 80.0% and 66.7% ; in 45 cancer adjacent tissues, the positive expression rates of Cox-2 and Ang-2 were 35.6% and 11. 1% , respectively. The positive expression rates of both Cox-2 and Ang-2 in eolorectal cancer tissues were significantly higher than those in cancer adjacent and normal colorectal tissues. There were close correlations between the expressions of Cox-2 and Ang-2 and some pathologic features, such as lymph node metastasis and Dukes stage; whereas there were no significant association between the expressions and gender, histological type and position of tumor. There was also a close correlation between the expressions of Cox-2 and Ang-2 themselves. Conclusion Cox-2 and Ang-2 play an important role in the occurrence and development of colorectal cancer. The use of specific inhibitor of Cox-2 as a treatment for colorectal cancer may become feasible

关 键 词：大肠癌 环氧合酶-2 促血管生成素-2 

分 类 号：R735.34[医药卫生—肿瘤]