糖基化终产物对人肾系膜细胞MMP-2表达的影响  

Effects of advanced glycosylation end products on the expression of MMP-2 in human renal mesangial cells

作　　者：杨益宁[1] 孙子林[1] 杨涛[1] 马婵娟[1] 马里[1] 刘乃丰[1] 

机构地区：[1]东南大学临床医学院,江苏南京210009

出　　处：《东南大学学报（医学版）》2006年第5期349-352,共4页Journal of Southeast University(Medical Science Edition)

基　　金：江苏省教育厅高校自然科学研究项目(02KJA320001);东南大学新进博士科研启动基金资助项目(9290002258)

摘　　要：目的:观察糖基化终产物(advanced glycosylation end products,AGEs)对人肾系膜细胞(hum an renal m esangial cell,HRMC)基质金属蛋白酶-2(m atrix m etalloprote inase,MMP-2)表达的影响。方法:运用糖基化修饰的牛血清白蛋白(AGE-BSA)干预体外培养的HRMC,W estern b lot检测系膜细胞MMP-2蛋白表达。结果:HRMC的MMP-2蛋白表达水平随AGE-BSA浓度增加和干预时间延长而降低(P<0.01)。结论:MMP-2可能参与AGEs所致的糖尿病肾病的发生发展。Objective To observe the effects of advanced glycosylation end products（AGEs） on the expression of matrix metalloproteinase-2（MMP-2） in cuhured human renal mesangial cells （HRMC）. Methods HRMC were incubated with different concentrations of AGE-BSA for different time. The protein expression of MMP-2 in HRMC were analyzed by Western blot. Result AGE-BSA decreased the expression of MMP-2 in HRMC time and dose dependently （ P 〈 0. 01 ）. Conclusion The data indicates that MMP-2 might mediate the role of AGEs in the development of diabetic nephropathy.

关 键 词：基质金属蛋白酶 人肾系膜细胞 糖基化终产物 糖尿病肾病 

分 类 号：R578.102[医药卫生—消化系统] R-33[医药卫生—内科学]