非小细胞肺癌组织中HIF-1α和MRP1、GST-π基因的表达及意义  

Expressions of HIF-1α,MRP1,and GST-π genes in non-small cell lung cancer tissue and their significances

作　　者：张咏梅[1] 宋光耀[1] 张淑慧[1] 王智华[1] 张文杰[1] 

机构地区：[1]河北省人民医院老年病重点实验室,石家庄050051

出　　处：《肿瘤》2006年第9期856-859,共4页Tumor

摘　　要：目的:探讨低氧诱导因子-1(HIF-1α)和多药耐药相关蛋白(MRP1)、谷胱甘肽-S-转移酶(GST-π)在肺癌组织中的表达水平以及与肺癌生物学特性的关系。方法:采用PT-PCR、凝胶电泳半定量方法检测36例非小细胞肺癌(non-small celllung cancer,NSCLC)组织中HIF-1αmRNA、MRP1 mRNA和GST-πmRNA的表达,并采用SAS 6.12统计软件分析它们在不同TNM分期中的表达情况。结果:随着TNM分期的增高,HIF-1αmRNA的表达显著性增高(P<0.01),MRP1 mRNA和GST-πmRNA表达均有增高趋势,但无统计学意义(P>0.05);三者在有淋巴结转移组和有远处转移组中的表达均明显高于无淋巴结转移组和无远处转移组(P<0.01,P<0.05)。HIF-1αmRNA的表达与MRP1 mRNA和GST-πmRNA的表达呈正相关。结论:HIF-1α,MRP-1和GST-π基因表达的增高可能与肿瘤组织的恶化有关。Objective： To investigate the expressions of hypoxia-inducible factor-1α（HIF-1α）, multidrug resistance related protein （MRP1）, and glutathione ＆transferase （GST-π） in non-small cell lung cancer （NSCLC） tissue and explore their rela tionship with the biological features of lung cancer. Methods：Reverse transcriptase-polymerase chain reaction and quasi-quantity gel electrophoresis were used to detect the mRNA expressions of H1F-1α, MRP1, and GST-α genes in 36 samples of NSCLC tissue. SAS6. 12 statistics software was used to analyze their expressions in different TNM stages. Results： The expression of HIF- 1α mRNA significantly increased accompanied with the elevation of TNM stage （P〈0.01）. The expressions of MRP1 and GST- π mRNA tended to increase, but the difference was not significant （P〉0.05）. The expressions of the three genes were significantly higher in the groups with lymph node metastasis and distant metastasis than that without lymph node metastasis and dis tant metastasis （P〈0.01 or P〈0.05）. The mRNA expression of HIFla had positive correlations withMRP1 and GST-π（P〈 0.05）. Conclusion： The over expression of HIF-1α, MRP-1, and GST-ngenes may he related with the deterioration of the NSCLC.

关 键 词：癌 非小细胞肺 低氧诱导因子 多药抗药相关蛋白质类 谷胱甘肽转移酶类 

分 类 号：R734.2[医药卫生—肿瘤]