The Dynamic Change of TGF-β_1 in the Myocardial Remodeling of Rat after Myocardial Infarction  

作　　者：郭张强 廖玉华 程翔 李彬 刘英 葛洪霞 张玲 王敏 郭和平 

机构地区：[1]Laboratory of Cardiovascular Immunology,Institute of Cardiology,Union Hospital,Tongji Medical College of Huazhong University and Technology,Wuhan 430022,China

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2006年第4期417-420,共4页华中科技大学学报（医学英德文版）

基　　金：This project was supported by a grant from National Natu-ral Sciences Foundation of China (No. 30370574)

摘　　要：Summary： To observe the dynamic changes of the TGF-β1 expressed in the infarct and non-infarcted region of rat heart during the ventricular remodeling （day 3, 7, 28, 180）, myocardial infarction rat model was made and relationship between the cytokine and indicator of myocardial remodeling was analyzed. After the detection of hemodynamic parameter was performed by the Powerlab devices, the size of myocardial infarction and the morphology change was detected by TTC and HE, respectively. The relative levels of mRNA of TGF-β 1, collagen type Ⅰ , Ⅲ, and fetal gene beta-MHC were detected by RT-PCR. The distribution of TGF-β1 protein in the myocardium was detected by immunohistochemistry. The results showed that the size of infarction was higher than that of the sham operated groups in the infarcted group （44.5±0.5 vs 0）. The difference in hemodynamic parameters between the infarcted group and sham operated group was significant （P〈0.01）. HE staining showed that inflammatory cells were accumulated in the infarcted region at the beginning of the 3^rd day, which lasted 4 weeks. Then, it decreased gradually. β-MHC in the non-infarcted region rose from the 3^rd day, reaching its peak at the 4^th week, and it decreased gradually. The ratio of the collagen type Ⅰ /Ⅲ showed similar changes as compared with the sham operated groups （P〈0.01）. And the relative mRNA levels in the non-infarcted group were significantly higher than that in the infarcted and sham operated group （P〈0.01） at day 180. Linear regression analysis indicated that the TGF-β1 was positively correlated with the ventricular remodeling. It was concluded that the cytokine TGF-β1 participates in the process of the myocardial remodeling, which could be a strategy in the interference of myocardial remodeling.Summary： To observe the dynamic changes of the TGF-β1 expressed in the infarct and non-infarcted region of rat heart during the ventricular remodeling （day 3, 7, 28, 180）, myocardial infarction rat model was made and relationship between the cytokine and indicator of myocardial remodeling was analyzed. After the detection of hemodynamic parameter was performed by the Powerlab devices, the size of myocardial infarction and the morphology change was detected by TTC and HE, respectively. The relative levels of mRNA of TGF-β 1, collagen type Ⅰ , Ⅲ, and fetal gene beta-MHC were detected by RT-PCR. The distribution of TGF-β1 protein in the myocardium was detected by immunohistochemistry. The results showed that the size of infarction was higher than that of the sham operated groups in the infarcted group （44.5±0.5 vs 0）. The difference in hemodynamic parameters between the infarcted group and sham operated group was significant （P〈0.01）. HE staining showed that inflammatory cells were accumulated in the infarcted region at the beginning of the 3^rd day, which lasted 4 weeks. Then, it decreased gradually. β-MHC in the non-infarcted region rose from the 3^rd day, reaching its peak at the 4^th week, and it decreased gradually. The ratio of the collagen type Ⅰ /Ⅲ showed similar changes as compared with the sham operated groups （P〈0.01）. And the relative mRNA levels in the non-infarcted group were significantly higher than that in the infarcted and sham operated group （P〈0.01） at day 180. Linear regression analysis indicated that the TGF-β1 was positively correlated with the ventricular remodeling. It was concluded that the cytokine TGF-β1 participates in the process of the myocardial remodeling, which could be a strategy in the interference of myocardial remodeling.

关 键 词：myocardial remodeling fetal gene CYTOKINE 

分 类 号：R542.22[医药卫生—心血管疾病]