CXCL12/CXCR4 Expression in Trophoblasts Takes Part in Materno-fetal Immune Tolerance and Vascular Remodeling  

作　　者：杨昀 邹丽 李梅 赵茵 

机构地区：[1]Department of Obstetrics and Gynecology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2006年第4期466-468,共3页华中科技大学学报（医学英德文版）

摘　　要：Summary： In this study, we investigated the expression of CXCL12 （SDF-1）/CXCR4 in trophoblasts and the role they play in the gestation. Immunochemistry was used to detect the expression of CXCR4 and CXCLI 2 in human villi and placenta. Highly purified extra-viUous trophoblasts （EVTs） ere detected for CXCR4 and CXCL12 in vitro by immunocytochemistry. The chemotaxis of CXCL12 was tested in transweU and the chemotactic activity was quantitatively examined. It was suggested that both CXCR4 and CXCL12 were expressed in trophoblasts and were decreased with the gestation time P〈0.05）. In a certain coverage, CXCL12 exhibited chemotactic activity which was positively correlated with its concentration [（r）=0.68, P〈0.01], the maximum chemotactic index （CI） was 1.62±0.12. Our results suggest that interaction between CXCR4 and CXCL12 is involved in materno-fetal immunological tolerance in all three trimesters of gestation and contributes to the invasion of EVTs during pregnancy.Summary： In this study, we investigated the expression of CXCL12 （SDF-1）/CXCR4 in trophoblasts and the role they play in the gestation. Immunochemistry was used to detect the expression of CXCR4 and CXCLI 2 in human villi and placenta. Highly purified extra-viUous trophoblasts （EVTs） ere detected for CXCR4 and CXCL12 in vitro by immunocytochemistry. The chemotaxis of CXCL12 was tested in transweU and the chemotactic activity was quantitatively examined. It was suggested that both CXCR4 and CXCL12 were expressed in trophoblasts and were decreased with the gestation time P〈0.05）. In a certain coverage, CXCL12 exhibited chemotactic activity which was positively correlated with its concentration [（r）=0.68, P〈0.01], the maximum chemotactic index （CI） was 1.62±0.12. Our results suggest that interaction between CXCR4 and CXCL12 is involved in materno-fetal immunological tolerance in all three trimesters of gestation and contributes to the invasion of EVTs during pregnancy.

关 键 词：CHEMOKINE chemokine receptor VILLI PLACENTA extra-villous trophoblasts 

分 类 号：R392[医药卫生—免疫学]