用基因芯片筛选胃黏膜癌变相关基因  

Screening of carcinogenesis associated genes in gastric carcinoma by gene chip

作　　者：许沈华[1] 余传定[1] 牟瀚舟[1] 姜志明[1] 朱赤红[1] 刘祥麟[1] 

机构地区：[1]浙江省肿瘤研究所,杭州310022

出　　处：《中华胃肠外科杂志》2006年第5期436-440,共5页Chinese Journal of Gastrointestinal Surgery

摘　　要：目的用基因芯片技术筛选与胃黏膜癌变相关的基因。方法利用Affymetrix公司生产的U133A基因芯片,对癌旁黏膜和切缘正常胃黏膜基因表达谱进行检测,并利用生物信息学方法对检测结果进行分析。结果(1)癌旁黏膜与正常胃黏膜比较差异3倍以上共有150个基因,其中表达上调[SLR(信号比的对数值)＞1．5]有130个,表达下调(SLR＜-1．5)有20个。从表达差异的基因功能分类看,以酶和酶调控子活性改变最多(28,占18．7%)；其次是与核酸结合活性相关基因(17,占11．3%)；第三是与信号传导活性相关基因(15,占10%)；第四是与蛋白结合活性相关基因(13,占8．7%)。除了40个功能未知的基因占总数26．7%外,以上4大类共占基因总数的48．7%。(2)癌旁黏膜(P)和胃癌(T)同时与正常胃黏膜比较,有71个相同的基因表达差异。其中表达上调(癌旁上皮SLR＞1．5)有61个,表达下调(癌旁上皮SLR＜-1．5)有10个。71个同时出现癌旁黏膜和胃癌的基因在染色体定位,发现19号染色体异常基因最多有11个；其次是1、2、16、17号染色体,分别是6个；5、14、22号和y染色体未发现异常的基因。结论71个癌旁黏膜与胃癌同时出现、表达相同的基因可能与早期胃癌的演化有关。酶和酶调控子活性、核酸结合活性、信号传导活性和蛋白结合活性4大类相关基因是研究胃癌发生的重要基因。19号染色体及1、2、16、17号染色体是研究胃癌发生的重要基因位点。Objective To screen the carcinogenesis associated genes in gastric carcinoma by gene chip. Methods UI33A （Affymetrix Santa Clara, CA） gene chip was used to detect differentially expressed genes in tumor tissues, paratumor mucosa and normal mucosa. Bioinformatics was used to analyze the screened results. Results A total of 150 genes were detected with a difference of expression levels more than 3 times in paratumor mucosa compared with normal gastric mucosa, 130 of which were up-regulated,and 20 down-regulated. According to the function classifications of the differentially expressed genes, the most common ones were enzyme and enzyme regulaon activity associated genes（28, 18.7% ）. The frequencies of nuclei acid binding activity associated genes, signal transduction associated genes and protein binding associated genes were 11.3%, 10%, and 8.7% respectively. Seventy-one differentially expressed genes were detected both in tumor tissues and paratumor mucosa compared with normal mucosa, 61 of which were up-regulated and 10 down-regulated. Among these 71 genes, eleven genes were localized on chromosome 19, 6 on chromosome 1, 2, 16, 17 respectively. No abnormal differentially expressed gene weres detected on chromosome 5, 14, 22 and Y. Conclusions These 71 genes differentially expressed both in tumor tissues and paratumor mucosa may be associated with carcinogenesis of gastric carcinoma. The four kinds of genes associated with enzyme and enzyme regulaon activity, nuclei acid binding activity, signal transduction, and protein binding should be the main genes for the study of carcinogenesis in gastric carcinoma.

关 键 词：基因芯片 基因表达谱 胃肿瘤 癌旁黏膜 

分 类 号：R735.2[医药卫生—肿瘤]