亚低温减少和延迟缺血再灌注后沙土鼠海马CA_1区细胞凋亡  

Mild hypothermia reduces and delays cell apoptosis in the hippocampal CA_1 region following ischemia-reperfusion injury

作　　者：张付生[1] 张刚[2] 董瑞国[3] 

机构地区：[1]山东省泰安市中心医院神经内科,泰安271000 [2]山东省泰安市中心医院心外科,泰安271000 [3]徐州医学院附属医院神经内科

出　　处：《中华物理医学与康复杂志》2006年第9期584-587,共4页Chinese Journal of Physical Medicine and Rehabilitation

摘　　要：目的研究亚低温对沙土鼠全脑缺血再灌注损伤海马CA_1区细胞的保护作用,并探讨其机制。方法采用沙土鼠全脑缺血再灌注损伤模型,用免疫组织化学方法,动态观察Bax、细胞色素C(Cyt C)、TUNEL阳性细胞等在常温组、亚低温组及假手术组的表达和变化。结果与常温组相比,亚低温组CA_1区各时间点凋亡细胞数显著减少,且其凋亡高峰延迟。Bax在3h、6h、1d3个时间点CA_1区表达较常温组明显减少,且表达高峰延迟,CytC的动态变化与Bax-致。结论亚低温能减少和延迟细胞凋亡,其机制可能与降低并延迟促凋亡蛋白Bax的表达和线粒体释放Cyt C有关。Objective To investigate the protective effects of mild hypothermia on cells in the CA1 region of the hippocampus in gerbils following global ischemia-reperfusion injury （IRI）, and to explore their mechanism. Methods IRI models were established in 75 gerbils. Any changes in TUNEL positive cells and the expression of Bax and Cytochrome C were then observed in normothermic （N） , hypothermic （H） and sham （S） groups through immunohistochemistry methods. Results In the H group （as compared with the N group） apoptotic cells in the CA1 subfield of the hippocampus significantly decreased. The expression of Bax and Cyt C at 3 h, 6 h and 1 d were decreased, and the expression fastigium was delayed. Conclusion Mild hypothermia can moderate and delay cell apoptosis, and its mechanism might be related with reducing and delaying the expression of Bax and Cyt C released by mitochondria.

关 键 词：亚低温 凋亡 Bax 细胞色素C 

分 类 号：R743[医药卫生—神经病学与精神病学]