丙型肝炎及其肝硬化组织中病毒核心蛋白及p53和Bcl-2的表达与相关性研究  

Hepatitis C virus core protein effects on expression of mutation p53 and Bcl-2 protein in hepatitis C virus hepatitis and cirrhosis

作　　者：张志培[1] 王文亮[2] 江逊[1] 赵佐庆[1] 成胜权[2] 郭双平[2] 

机构地区：[1]第四军医大学唐都医院实验外科,陕西西安710032 [2]第四军医大学基础部病理教研室,陕西西安710032

出　　处：《医学研究生学报》2006年第9期771-773,776,i0007,共5页Journal of Medical Postgraduates

基　　金：国家自然科学基金资助项目(批准号:30000076)

摘　　要：目的:探讨丙型肝炎及其肝硬化组织中丙型肝炎病毒(HCV)核心蛋白、突变p53、Bc l-2的表达及其相关性,探讨HCV核心蛋白是否能促进p53突变和Bc l-2的表达。方法:收集HCV表达阳性的肝炎、肝硬化组织23份,采用免疫组化Envision法检测HCV核心蛋白、突变p53、Bc l-2的表达,并分析三者间的关系。结果:核心蛋白和突变p53的阳性表达主要定位于细胞核中,Bc l-2的阳性表达主要定位于细胞质中;在核心蛋白表达阳性的组织中,突变p53的阳性表达率为87.0%,Bc l-2的阳性表达率为95.7%,三组间阳性强度的差异无显著性意义(P=0.095);HCV-C蛋白与突变p53、Bc l-2阳性强度两者间相关性检验的P值分别为0.011和0.012,相关系数r分别为0.69和0.72;突变p53与Bc l-2两者相关性检验P=0.007,r=0.72。结论:三种蛋白的表达有相关性;HCV核心蛋白可能促进野生p53突变;核心蛋白和突变p53都有可能促进Bc l-2蛋白的表达。Objective： To explore the expression of H CV core, mutation p53, and Bcl-2 proteins, and the correlation of these three proteins in the tissues of HCV infection or cirrhosis; to explore weather HCV core protein promotes the production of mutation p53 and/or the expression of Bcl-2 protein. Methods ： Collect tissues from 23 HCV infected patients and determine the expression of HCV core, p53, and Bcl-2 proteins with an immunohistochemical method （ Envision method） ; analyze the correlation of the three proteins by statistics. Results ：The positive expression of HCV core and mutation p53 proteins primarily lay in the nucleus, while the positive expression of Bcl-2 protein lay in the cytoplasm; the posi- tive rate of mutation p53 was 87.0%, while the positive rate of Bcl-2 was 95.7% in the tissues with HCV core positive expression. There was no difference （P = 0.095 ） in expression of the three proteins; P value of correlationship test of positive intensity between HCV core and p53, HCV core and Bcl-2 was respectively 0.011 and 0.012, while the correlation coefficient was respectively 0.69 and 0.72 ; and P value of correlation test of positive intensity between mutation p53 and Bcl-2 was 0.007 with a correlation coefficient as 0.72. Conclusion ： The expression of the three proteins is correlated ： maybe the HCV core protein promotes mutation of wild p53; HCV core and mutation p53 proteins increase the expression of Bcl-2 protein.

关 键 词：丙型肝炎病毒核心蛋白 突变P53 BCL-2 丙型肝炎 肝硬化 

分 类 号：R735.7[医药卫生—肿瘤]