蟑螂变应原诱导小鼠肺部变应性炎症动物模型的研究  

Study on a murine model of cockroach allergen-induced allergic asthma

作　　者：许卓谦[1,2] 刘志刚[1] 于琨瑛[1] 喻海琼[1,2] 丘劲[1] 

机构地区：[1]深圳大学生命科学学院 [2]广州医学院,广州510182

出　　处：《免疫学杂志》2006年第5期491-495,共5页Immunological Journal

基　　金：国家自然科学基金(39860071;30571625);广东省科技计划重点项目(2003A3080502);深圳市科技计划项目(200326)资助

摘　　要：目的建立美洲大蠊提取液(CAE)诱导的小鼠变应性气道炎症动物模型。方法24只Balb/c小鼠随机分为正常对照组(C组)和美洲大蠊粗浸液2个剂量组低剂量50μg组(A组)、高剂量100μg组(B组),8只/组。通过HE染色和PAS染色观察小鼠肺部炎症和黏液分泌;观察支气管肺泡灌洗液中细胞总数和细胞分类;用酶联免疫吸附试验检测BALF、脾细胞培养上清液的细胞因子和血清CAE特异的IgE、IgG1、IgG2a抗体。结果A、B组与对照组C组比较,可诱导肺组织出现明显的气道变态反应性炎症,肺组织炎症病理评分有显著性差异(P<0.01);BALF中的细胞总数、EOS计数、中性粒细胞数、IL-4,血清抗原特异性IgE抗体、IgG1抗体和脾细胞分泌IL-4均显著高于正常对照组C组(P<0.01);且BALF中的嗜酸性粒细胞计数和IL-4水平与美洲大蠊粗浸液的使用剂量有关(P<0.01)。结论美洲大蠊提取液能成功建立小鼠变态反应气道炎症动物模型,且呈剂量依赖性,高剂量能更有效诱导气道变态反应性炎症。Objective To develop a murine model of allergic airway inflammation by using crude Periplaneta americana extract （CAE） as clinical relevant allergen. Methods A total of 24 Balb/c mice were divided randomly into 3 groups： control group （group C, n = 8）, low-dose CAE （50μg） group （group A, n = 8）, and high-dose CAE （100μg） group （group B, n = 8）. After sensitized by subcutaneous injection and challenged by intranasal instillation of CAE. The lungs of the mice were stained with haematoxylin and eosin （H＆E）. The lung tissues were also stained with periodic acid-Schiff （PAS） to evaluate the proliferation degree of goblet cells. The total cell number and composition of BALF samples were determined. The cytokines in splenocytes culture supematants and BALF were assayed by enzyme-linked immunoassay （ELISA）. CAE specific IgG1, IgG2a, and IgE in the serum were also measured by ELISA. Results Compared with mice in group C, mice in groups A and B developed signifieant abnormal reactive inflammation in airways. There was significant difference of histological scoring for pulmonary inflammation （P 〈 0.01） between mice in group C and mice in groups A and B. The total cells, eosinophils, neutrophils, and IL-4 in BALF, the allergen-specific antibody IgE and IgG1 in serum, and the IL-4 released from splenocytes into supernatants in vitro were all significantly increased in groups A and B compared with group C （P 〈 0.01）. Additionally, eosinophils and IL-4 levels in BALF of groups A and B were correlated to the dose of CAE. Conclusion CAE could successfully induce a murine model of allergic airway inflammation and the development of this model is dependent on the dose of CAE administration： high dose is more effective in inducing pulmonary allergic inflammation.

关 键 词：蟑螂变应原 动物模型 哮喘 

分 类 号：R-332[医药卫生]