反相高效液相色谱法测定茶叶中β-胡萝卜素含量  

Determination of Beta-carotene in Tea by Reverse High Performance Liquid Chromatography

作　　者：王新超[1] 陈亮[1] 赵丽萍[1] 马春雷[1] 张亚丽[1] 梅菊芬[1] 姚明哲[1] 

机构地区：[1]中国农业科学院茶叶研究所/农业部茶叶化学工程重点实验室,杭州310008

出　　处：《中国农学通报》2006年第9期91-93,共3页Chinese Agricultural Science Bulletin

基　　金：浙江省分析测试基金项目"茶叶胡萝卜素测定方法的建立与高含量茶树品种的初步筛选("04027)资助。

摘　　要：为了解茶叶中β-胡萝卜素(β-carotene)的含量,建立了用反相高效液相色谱测定茶叶中β-胡萝卜素含量的方法。样品先用丙酮浸提,真空旋转蒸干,再加乙醚和甲醇-氢氧化钾低温避光皂3h,然后用10%氯化钠萃取,乙醚相真空旋转蒸干,最后溶解于甲醇:乙酸乙酯混合液(1:1)。色谱方法如下:HypersilC18ODS柱,流动相为丙酮和水,梯度洗脱,检测波长为450nm。结果表明,该方法重现性好,平均加样回收率为99.2%,RSD为4.5%。用该方法测定了同一茶树品种不同部位叶片和不同季节一芽二叶的β-胡萝卜素含量,表明茶树叶片的β-胡萝卜素含量是成熟叶高于幼嫩叶片,夏秋季高于春季。A simple high performance liquid chromatographic analysis method of tea β-carotene has been developed. Firstly, samples were leached by acetone and reduced in the vacuum rotation evaporator. Secondly, samples were saponified by addition of diethyl ether and methanolic KOH, and allowed to rest for 3h at 4℃ with light excluded, and extracted by 10% NaCl, then the diethyl ether phases were reduced in the vacuum rotation evaporator. Finally, residues was solvated with methanol： ethyl acetate （v：v=1：1）. The separation system consisted of a C18 reversed-phase column, a gradient elution system of acetone and water and detection wavelength of 450 nm. While the content of β-carotene ranged between 2.5-100μ g/ ml, the method showed a good linear relationship between the peak area and content of β-carotene （r^2= 0.9936） and high reproducibility. The average rate of recovery of β-carotene was 99.2 % and the relative standard deviation （RSD） was 4.5%. Beta-carotene contents of different positional and seasonal leaves of a tea variety Longjing 43 were measured using this method. It can be concluded that the content of β- carotene is higher in mature leaves than in immature leaves, summer and autumn shoots higher than spring shoots.

关 键 词：高效液相色谱法 Β-胡萝卜素 茶叶 含量测定 

分 类 号：Q562[生物学—生物化学] O657.72[理学—分析化学]