Ginkgo biloba extract suppresses hypertrophy and extracellular matrix accumulation in rat mesangial cells  

作　　者：Jian-yun WANG Xiao-xing YIN Yun-ming WU Dao-quan TANG Yuan-yuan GAO Mei-rong WAN Xiao-yu HOU Bei ZHANG 

机构地区：[1]Department of Pharmacy [2]Provincial Key Lab for Brain Diseases Bioinformation, Xuzhou Medical College, Xuzhou 221002, China [3]Center-Laboratory, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China [4]Department of Obstetrics and Gynecology, The Third People's Hospital of Xuzhou, Xuzhou 221002, China

出　　处：《Acta Pharmacologica Sinica》2006年第9期1222-1230,共9页中国药理学报（英文版）

基　　金：Project supported by College Natural Science Research Foundation of Jiangsu Province(№03KJ13360143).

摘　　要：Aim： To observe the effects of Ginkgo biloba extract （EGb） on the hypertrophy of mesangial cells and the accumulation of extracellular matrix （ECM） in mesangial cells. Methods： Cultured mesangial cells were allotted into 7 groups： normal group, solvent control group, high glucose group, low dose of EGb group, moderate dose of EGb group, high dose of EGb group, and captopril group. Activities of cell antioxidases, S phase percentage and G0/G1 phase percentage, collagen Ⅳ and laminin, Smad2/3 and Smad7, TGF-β1 mRNA were measured by different methods. Results： For EGb-treated groups, when compared with high glucose group, the cell percentage of S phase was raised and the percentage of G0/G1 was lowered. The intensity of oxidative stress was weakened. The expression of Smad2/3 was greatly decreased and Smad7 was increased. Collagen Ⅳ, laminin and TGF-β1 mRNA were also reduced. Conclusion： EGb can suppress cell hypertrophy and the accumulation of ECM in rat mesangial cells, which means it could play a vital role in the delay of glomerulosclerosis in diabetic nephropathy.Aim： To observe the effects of Ginkgo biloba extract （EGb） on the hypertrophy of mesangial cells and the accumulation of extracellular matrix （ECM） in mesangial cells. Methods： Cultured mesangial cells were allotted into 7 groups： normal group, solvent control group, high glucose group, low dose of EGb group, moderate dose of EGb group, high dose of EGb group, and captopril group. Activities of cell antioxidases, S phase percentage and G0/G1 phase percentage, collagen Ⅳ and laminin, Smad2/3 and Smad7, TGF-β1 mRNA were measured by different methods. Results： For EGb-treated groups, when compared with high glucose group, the cell percentage of S phase was raised and the percentage of G0/G1 was lowered. The intensity of oxidative stress was weakened. The expression of Smad2/3 was greatly decreased and Smad7 was increased. Collagen Ⅳ, laminin and TGF-β1 mRNA were also reduced. Conclusion： EGb can suppress cell hypertrophy and the accumulation of ECM in rat mesangial cells, which means it could play a vital role in the delay of glomerulosclerosis in diabetic nephropathy.

关 键 词：ginkgo biloba extract GLOMERULOSCLEROSIS oxidative stress TGF-β1 SMADS 

分 类 号：R692[医药卫生—泌尿科学]