沙培林局部应用治疗鼠脑胶质瘤电镜观察  

The Electro-microscope View of Cerebral Glioma of Nude Mice Treated with Regional Application of Sapylin

作　　者：聂振明[1] 赵长地[1] 聂亚一[2] 邓连夫[3] 卢尚佩 

机构地区：[1]济宁医学院附属第一人民医院 [2]上海复旦大学生物医学研究院 [3]上海瑞金医院骨科研究所 [4]山东鲁抗医药集团动物实验室

出　　处：《济宁医学院学报》2006年第3期17-18,共2页Journal of Jining Medical University

摘　　要：目的探讨沙培林局部应用治疗裸鼠脑胶质瘤细胞形态学的改变。方法BALB／C裸鼠16只，接种瘤细胞成功后随机分成4组，每组4只，接种后10d肿瘤长至约3～4mm大小时开始给药，对照组0．2ml生理盐水，低剂量组沙培林0．2KE，中剂量组0．4KE，高剂量纽0．8KE，瘤周注射，隔日1次，给药8次后解瘤实验。标本经前固定、漂洗、后固定、漂洗、脱水、置换、浸透、包埋、超薄切片机切片等处理，用HITACHIH（日本日立公司）H～500透射电镜观察。结果对照组电镜下见正常的胶质瘤细胞，偶可见空泡变性，而无坏死或凋亡；低、中、高剂量组，电镜下均可见到瘤细胞有核固缩及凋亡小体的出现。结论从病理形态学上证实了沙培林局部应用于鼠脑胶质瘤，可抑制脑胶质瘤的生长，促进肿瘤细胞的凋亡。Objective To investigate the morphological changes of cerebral glioma cells of nude mice treated with local application of Sapylin. Methods 16 post hypodermic vaccinial BALB/C nude mouse with U251 glioma were randomly divided into 4 groups, with 4 members each groups.We began to administer drug when glioma growed into 3mm × 4mm size with 10 days after vaccination. 0.2 ml saline solution to comparison group, 0.2 KE Sapylin to low dosage group, 0.4 KE Sapylin to medium dosage group, 0.8KE Sapylin to high dosage group,with tertian injection to the periphery of tumors.After 8 times, tumors were dissected.After pre- fixation, washing, post - fixation, dehydration, permutation, saturation, embedding and making sections, then ob- served under HITACHIH H- 500 transmission microscope. Results There are normal glioma cells under electro- microscope in compared groups, with occasional vacuolar degeneration. Whereas we can see pyknosis and apoptotic body in low - dose group, middle - dose group and high - dose group. Conclusion We can prove in pathologic morphologic way that Sapylin can inhibit glioma growth and raising expressions of apoptosis.

关 键 词：沙培林 局部应用 ALB/C裸鼠 电镜观察 

分 类 号：R739.8[医药卫生—肿瘤]