响应面分析法优化固定化细胞转化DL-ATC生产L-半胱氨酸工艺条件  

The optimization of bioconversion conditions for the production of L-cysteine with immobilized cells based on response surface analysis

作　　者：王普[1] 王浩[1] 张世杰[1] 张晓军[1] 

机构地区：[1]浙江工业大学药学院,浙江杭州310032

出　　处：《浙江工业大学学报》2006年第4期355-359,共5页Journal of Zhejiang University of Technology

摘　　要：利用SAS软件的Plackett-Burman试验设计法及响应面分析法,对海藻酸钙固定化假单胞菌(Pseudomonas sp.)HY1040转化DL-2-氨基-△2-噻唑啉-4-羧酸(DL-ATC)生产L-半胱氨酸的工艺条件进行了优化,得到较佳的转化工艺条件:固定化细胞接种量为25.5 mL,DL-ATC质量浓度为1.0%,固定化细胞增殖时间为12.9 h.经5批次转化试验考察,固定化细胞比酶活力平均达934u/mL,较优化前提高38.9%.经连续转化4批次,底物转化率仍可保持在初始值的91.0%以上.The conversion conditions for the production of L-cysteine using immobilized cells were optimized by using SAS software combined with the method of Plackett-Burman design and the Response Surface Analysis. The cells were immobilized by calcium alginate embedding method. The optimum levels of three important factors were determined as follows： the volume of immobilized gel was 25.5 mL, the concentration of DL-ATC was 1.0% （wt） and the generative time of immobilized cells was 12.9 h. Experiments showed that the average enzyme activity could reach 934 u/mL at optimized conditions for five batches, with an increase of 38.9% compared with that before the optimization. After the immobilized cells were utilized for 4 times, the conversion rate could be still over 91.0% of the initial value.

关 键 词：固定化细胞 DL—ATC 微生物转化 L-半胱氨酸 响应面分析 Plackett—Burman设计法 

分 类 号：Q93[生物学—微生物学]