坛紫菜单个体细胞培养的愈伤组织发育途径  

The Morphogeny of Callus from Single Somatic Cell Clone Cultivation of Porphyra Haitanensis

作　　者：胡银茂[1] 

机构地区：[1]宁波教育学院科技分院,浙江宁波315010

出　　处：《台州学院学报》2006年第3期67-69,共3页Journal of Taizhou University

摘　　要：用海螺酶酶解坛紫菜(Porphyra haitanensis)叶状体制备游离的营养细胞,在显微镜下选取单个细胞放入96孔板中进行隔离培养。一部分细胞发育成了愈伤组织状的细胞团。这些细胞团继续发育,最终形成了丝状体或小叶片。The releases of free - living vegetative ceils were achieved by enzymolysis of Porphyra haitanensis fronds, then single somatic cells were picked out into 96 - well microplates under invert microscope, with the help of capillary tube. The development of somatic ceils was observed continuously and photos were taken. In this paper, the morphology of callus that developed from one cell was described. The final destiny of callus was conchocelia or small blades.

关 键 词：坛紫菜 单细胞 愈伤组织 

分 类 号：S968.43[农业科学—水产养殖]