阿米诺利敏感的小鼠生精细胞离子电流  

Currents Sensitive to Amiloride in Mouse Spermatogenic Cells

作　　者：王昌松[1] 高晓华[1] 陆亮[1] 龚伟[2] 程洁[1] 高蓉[1] 肖杭[1] 

机构地区：[1]南京医科大学应用毒理省重点实验室,南京210029 [2]江苏省疾病控制中心,南京210009

出　　处：《实验动物与比较医学》2006年第3期131-133,139,共4页Laboratory Animal and Comparative Medicine

基　　金：国家自然科学基金资助项目(30571555);国家973基础研究重大资助项目(2002CB512908)

摘　　要：目的研究记录小鼠生精细胞上表皮钠通道电流。方法应用机械分离获得小鼠单个生精细胞,采用全细胞膜片钳技术记录上皮钠离子通道电流。结果当钳制电位在-50mV、指令电压-100～+40mV、步阶电压10mV,测试脉冲为200ms时,可记录到一快速激活并且不失活的电流。细胞外液中加入1μmol/L的阿米诺利能显著性抑制该电流,并呈现出两类不同的作用(抑制率分别为58.49±9.82%,31.44±5.69%)。结论电流特性和阿米诺利敏感性特征表明,生精细胞上所记录到的电流是上皮钠通道电流。Objective To observe epithelial sodium channels （ENaC） currents in spermatogenic cells. Method Epithelial sodium channels were obtained in acutely dissociated mouse spermatogenic cells using whole-cell patch clamp. Results The currents were evoked by 200 ms test pulses between -100-＋40 mV in 10 mV increments from a holding potential （HP） of-50 mV. Test pulses elicited rapidly activating and non-inactivating Na^＋ current. Na^＋ currents amplitude were significantly reduced by adding Amiloride （1 μmol/L） to the external solution, and displayed two kinds of inhibition effects （the ratio of 58.49%±9.82%, 31.44%±5.69%）. Conclusion The characteristics of such currents and sensitive to Amiloride all approved that these currents recorded from mouse spermatogenic cells were produced through epithelial sodium channels.

关 键 词：生精细胞 全细胞膜片钳技术 上皮钠通道 小鼠 

分 类 号：R-332[医药卫生]