垂直喉部分切除术后喉狭窄瘢痕组织中炎症细胞表达及意义  

Expression of Inflammatory Cells and Its Effects on Laryngeal Stenosis Scar after Vertical Partical Laryngectomy

作　　者：吴旋[1] 苏振忠[1] 蒋爱云[1] 古聪敏[2] 赖英荣[2] 雷文斌[1] 蔡谦[1] 

机构地区：[1]中山大学附属第一医院耳鼻喉科医院,广东广州510080 [2]中山大学附属第一医院病理科,广东广州510080

出　　处：《中山大学学报（医学科学版）》2006年第5期545-548,共4页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广东省自然科学基金资助项目(31733)

摘　　要：【目的】通过喉狭窄瘢痕组织的病理学研究,了解炎症细胞在狭窄瘢痕组织表达及意义。【方法】垂直喉部分切除术后2～3月出现喉狭窄10例和未狭窄16例喉瘢痕肉芽组织为实验组及10例正常声带组织为对照组,分别进行苏木精-伊红及免疫组化染色,观察组织切片中中性粒细胞、巨噬细胞、T淋巴细胞表达,并在全自动图像分析系统下进行细胞计数。【结果】喉狭窄瘢痕组织与非狭窄喉瘢痕组织、正常声带比较,固有层炎症细胞大量聚集,主要以巨噬细胞和T淋巴细胞为主。比较非狭窄喉瘢痕组织及正常声带组织,喉狭窄瘢痕组织的中性粒细胞、巨噬细胞、T淋巴细胞数量均明显增加,差异有统计学意义(P<0.01)。【结论】喉狭窄瘢痕肉芽组织中存在明显炎症反应,持续炎症反应可能是导致喉狭窄瘢痕肉芽增生重要因素之一。[Objective] To explore the significance of the expression of inflammation cell in the laryngeal stricture scar by the study of the pathological features of laryngeal stenosis scar. [Methods] The laryngeal tissues were 10 human surgical specimens of laryngeal stenosis scar and 16 human surgical specimens of laryngeal scar which no-stenosis after vertical partial laryngectomy; 10 human normal vocal folds were control. Expression of neutrocytes, macrophage, and T lymphocyte were observed by Van Gieson and immunohistochemistry staining respectively in laryngeal tissue sections. The amount of inflammatory cells were analyzed with KONTRON IBAS 2.5 automatic image analysis system. [Results] The soakage density of neutrocytes, macrophage, and T lymphocyte were high in inhesion layer of laryngeal stenosis scar. The count of neutrocytes, macrophage, and T lymphocyte in laryngeal stenosis group were significantly higher than those in the no-stenosis scar group and normal vocal folds group （P 〈 0.01）. [Conclusion] There were obvious inflammation reaction in the laryngeal stenosis scar. Persistence inflammation reaction may play an important role in the hyperplasia of laryngeal stenosis scar.

关 键 词：垂直喉部分切除术 喉狭窄/病理学 炎症细胞 

分 类 号：R767[医药卫生—耳鼻咽喉科]