精子蛋白17在上皮性卵巢癌中的异常表达  

Aberrant expression of sperm protein 17 in epithelial ovarian cancer

作　　者：李芳秋[1] 杨爱龙[1] 黄文斌[2] 李南云[2] 曾素 

机构地区：[1]南京军区南京总医院全军医学检验中心,江苏南京210002 [2]南京军区南京总医院病理科,江苏南京210002 [3]南京军区南京总医院妇产科,江苏南京210002

出　　处：《中华肿瘤防治杂志》2006年第15期1174-1177,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：江苏省"六大人才高峰"项目基金资助(2005A2)

摘　　要：目的:探讨精子蛋白17(sperm pro-tein17,Sp17)在各类卵巢恶性肿瘤中的表达谱,并分析其意义。方法:用免疫组化技术检测65例卵巢恶性肿瘤组织标本Sp17的表达。结果:41例原发上皮性卵巢癌组织切片,18例不同程度地显示Sp17的异常表达,阳性率为43·9%;免疫细胞化学染色还在卵巢癌患者腹水中发现Sp17阳性癌细胞。7例卵巢原发的非上皮性恶性肿瘤和17例卵巢转移癌组织切片(原发癌来源于胃、结肠、输卵管、胆囊和肺等器官),免疫组化染色均未检出Sp17。结论:Sp17在原发上皮性卵巢癌中常见蛋白质水平的异常表达,在其他卵巢恶性肿瘤中未见表达,有可能作为上皮性卵巢癌新的诊断标志和免疫治疗靶标。OBJECTIVE： To explore the expression profile and significance of sperm protein 17 （Spl7） in variant ovarian tumors. METHODS：Immunohistochemical staining （IHC） was used to detect the expression of Spl7 in 65 tissue samples of ovarian tumor. RESULTS= Eighteen of 41 （44%） epithelial ovarian cancer tissue sec tions showed Spl7 expression at different degree. By using immunocytochemistry staining, Spl 7-positive cancer cells were found in ascites fluid from patients with ovarian cancer. None of 7 no-epithelial tumors primarily developed in ovarian and 17 ovarian adenocarcinoma metastatic （primary cancers in stomach, colon, oviduct, cholecyst, lung and other organ） were determined to be Sp17 positive IHC. CONCLUSIONS= Sp17 is expressed frequently at the protein level in epithelial ovarian cancers and thus represents a novel diagnostic molecular marker and an immunotherapy target for epithelial ovarian cancer.

关 键 词：卵巢肿瘤/病理学 精子 克隆 分子 重组融合蛋白质类/遗传学 免疫组织化学 

分 类 号：R737.31[医药卫生—肿瘤]