大鼠局灶性脑缺血再灌注血浆细胞因子和内皮素对红景天干预的反应  

Interventional effects of gold theragran on plasma levels of cytokine and endothelin in rats with focal cerebral ischemia-reperfusion injury

作　　者：隋汝波[1] 李熙东[1] 刘媛媛[1] 杨波[1] 闵连秋[1] 

机构地区：[1]锦州医学院附属第一医院神经内科,辽宁省锦州市121001

出　　处：《中国临床康复》2006年第35期23-25,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:观察大鼠局灶性脑缺血再灌注损伤时血浆细胞因子和内皮素含量的变化,及红景天对其变化的影响。方法:实验于2005-10/12在锦州医学院药理教研室进行。取30只SD大鼠随机数字法等分为3组:①假手术组:生理盐水按2mL/d灌胃5d后手术操作同其他组,但不夹闭右侧颈总动脉。②局灶性脑缺血再灌注模型组:生理盐水按2mL/d灌胃5d后,用10g/L戊巴比妥纳(40mg/kg)麻醉大鼠,取颈正中切口,暴露右侧颈总动脉、颈内动脉和颈外动脉,结扎颈总动脉、颈外动脉,于颈总动脉分叉下方剪一小口,将预先烧成圆头的4-0单丝尼龙缝线经该切口插入颈内动脉约(18±1)mm,将大脑中动脉起始部阻塞,1h后将栓线抽出约1cm,恢复再灌注1h。③红景天组:红景天按2mL/d灌胃给药,连续5d后与局灶性脑缺血再灌注损伤组相同。③假手术组及局灶性脑缺血再灌注损伤组、红景天组再灌注1h后均立即取血,利用放射免疫技术检测大鼠血浆细胞因子肿瘤坏死因子α、白细胞介素6、内皮素的水平。结果:30只大鼠全部进入结果分析。①脑局灶性缺血再灌注损伤组大鼠血中细胞因子肿瘤坏死因子α、白细胞介素6及内皮素含量较假手术组明显增高[(1.40±0.14),(0.68±0.10)mg/Le(142.60±12.33),(78.60±6.42)μg/Le(168.40±3.87),(114.50±2.84)μg/L,P<0.05]。②红景天组血中细胞因子肿瘤坏死因子α、白细胞介素6、内皮素含量较局灶性缺血再灌注组下降[(1.02±0.20),(1.40±0.14)mg/Le(121.00±13.96),(142.60±12.33)μg/Le(155.00±5.88),(168.40±3.87)μg/L,P<0.05]。结论:红景天显著降低局灶性脑缺血再灌注损伤大鼠血中细胞因子及内皮素水平,红景天的保护效应部分是由于减少血浆细胞因子肿瘤坏死因子α、白细胞介素6和内皮素的水平。AIM： To observe the changes in content of endothelin （ET） and plasma cytokines in rats with focal cerebral ischemia-reperfusion injury as well as the effects of gold theragran on its changes. METHODS： The experiment was conducted at the Pharmacological Department of Jinzhou Medical College between October and December 2005. Thirty SD rats were selected and randomly divided into 3 groups： ①Sham-operation group： rats were given gastric perfusion of normal saline at 2 mL/day for 5 days and then were operated the same as other groups without occluding the right common carotid artery （CCA）. ②Focal cerebral ischemia-reperfusion model group （model group）： rats were administrated with normal saline at 2 mL/day for 5 days, and then were anesthetized by 10 g/L carbrital （40 mg/kg） to make median incision on carotid so as to expose the left CCA, internal carotid （IC） and external carotid artery （ECA）. The ECA and CCA were deligated and a small incision was made in the furcation of CCA. A 4/0 monofilament nylon suture with probe-pointed was inserted via the incision along the CCA to （18±1 ） mm deep in the IC, so as to occlude the beginning of middle cerebral artery （MCA）. The suture was drew out for about 1 cm one hour later to recover the reperfusion for one hour.③Gold theragran group： Rats were administrated with gold theragran at 2 mL/day for 5 continuous days, which were the same as the model group.③Blood were collected from rats of three groups at one hour immediately after the reperfusion, and the plasma levels of tumor necrosis factor （TNF）-α, interleukin （IL）-6 and ET were detected by radioimmunoassay. RESULTS： A total of 30 rats were involved in the analysis of results.① The plasma levels of TNF-α, IL-6 and ET in rats of model group were higher than those in the sham-operation group [（1,40±0.14）, （0.68±0.10） mg/L; （ 142.60±12.33 ）, （78.60±.42）μg/L;（ 168.40±3.87）, （114.50±2.84） μg/L,P 〈 0,05],②The plasma levels of T

关 键 词：脑缺血 再灌注损伤 红景天属 细胞因子类 大鼠 

分 类 号：R742[医药卫生—神经病学与精神病学]