川芎嗪对脑缺血再灌注损伤后细胞粘附作用的影响(英文)  

Effect of ligustrazine on cell adhesion after cerebral ischemia-reperfusion injury

作　　者：高长越[1] 周华东[1] 邓娟[1] 普兴富 

机构地区：[1]解放军第三军医大学大坪医院脑血管病二科,重庆市400042 [2]玉溪市第二人民医院精神科,云南省玉溪市653100

出　　处：《中国临床康复》2006年第35期178-179,192,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:大脑缺血区白细胞与内皮细胞的活化及表面粘附分子的表达可使白细胞聚集浸润,发挥细胞毒作用进而使脑损伤加重。中药川芎嗪具有抗血栓形成,改善微循环的作用。目的:观察川芎嗪对脑缺血鄄再灌注损伤后局灶区白细胞与内皮细胞粘附性变化的影响。设计:随机对照动物实验。单位:第三军医大学大坪医院脑血管病二科,玉溪市第二人民医院精神科。材料:实验于第三军医大学大坪医院脑血管病二科实验室完成。雄性Wistar大鼠32只,体质量250～300g,随机分为3组,8只为正常对照组,12只为脑缺血鄄再灌注组,12只为脑缺血-再灌注加用川芎嗪组。方法:川芎嗪组静脉注射磷酸川芎嗪注射液(15g/kg)。正常对照组及脑缺血组予静脉注射等量生理盐水。通过免疫荧光标记技术和显微超高速录像系统观察脑缺血-再灌注及应用川芎嗪后局灶区白细胞粘附性的变化。主要观察指标:①脑缺血鄄再灌注损伤局灶区微动脉白细胞附壁密度指数及白细胞与内皮细胞之间黏附力。②应用川芎嗪后局灶区白细胞粘附性的变化。结果:实验动物全部进入结果分析。①与对照组比较,脑缺血鄄再灌注损伤组局灶区微动脉白细胞附壁密度指数增大,白细胞与内皮细胞间断裂应力明显下降,粘附力显著增强,差异均有显著性(P<0.01)。②与脑缺血鄄再灌注损伤组比较,川芎嗪治疗组白细胞附壁密度指数及粘附力显著下降,至24h后降至较低水平,断裂应力增高,至24h与正常对照组差异无显著性,并维持在较高水平。结论:川芎嗪可显著减轻脑缺血鄄再灌注损伤后内皮细胞与白细胞的粘附。BACKGROUND： Activation of leucocyte and endothelial cell in isehemie area of brain and the expression of adhesive molecule on the surface can cause aggregation and infiltration of leucocyte which deteriorated cerebral injury due to cytotoxicity. Ligustrazine can inhibit thrombosis and improve microcirculation. OBJECTIVE： To observe the effect of ligustrazine on adhesion of leucocyte and endothelial cell in focal area after cerebral ischemia-reperfusion injury. DESIGN： Randomized controlled animal study. SETTING： Second Division of Brain, Department of Neurology, Daping Hospital of the Third Military Medical University of Chinese PLA; Department of Psychiatry, the Second People＇s Hospital of Yuxi, Yunnan Province. MATERIALS： The experiment was carried out at the Second Cerebral Laboratory of Neurological Department of Daping Hospital Affiliated to the Third Military Medical University of Chinese PLA. A total of 32 male Wistar rats weighing 250-300 g were randomly divided into normal control group （n=8）, cerebral ischemia-reperfusion group （n=12） and cerebral ischemia-reperfusion ＋ ligustrazine group （n=12）. METHODS： Rats in ligustrazine group were venously injected with 15 g/kg ligustrazine phosphoric acid, and rats in normal control group and cerebral ischemia-reperfusion group were venously injected with the same volume of saline. Cerebral ischemia-reperfusion injury and adhesive changes of leucocyte in focal area after administration of ligustrazine were observed with immunofluorescent labeling technique and miero-uhra-speed imaging system. MAIN OUTCOME MEASURES： ① Adhesive density index of leucocyte of arteriole in focal area after cerebral ischemia-reperfusion injury; ② Adhesive changes of leucocyte in focal area after administration of ligustrazine. RESULTS： All experimental animals were involved in the final analysis. ① As compared with those in control group, adhesive density index of leucocyte of arteriole in focal area in cerebral isehemia-reperfusion group was incre

关 键 词：脑缺血 再灌注损伤 白细胞 内皮细胞 川芎嗪 胞间黏附分子 

分 类 号：R743[医药卫生—神经病学与精神病学]