平阳霉素乳剂涂擦治疗增生期毛细血管瘤30例  

Pingyangmycin Treating Infantile Proliferating Capillary Hemangiomas in 30 Children

作　　者：徐伟立[1] 牛爱国[1] 李英超[1] 

机构地区：[1]河北医科大学第二医院小儿外科,石家庄050000

出　　处：《实用儿科临床杂志》2006年第17期1190-1191,共2页Journal of Applied Clinical Pediatrics

摘　　要：目的探讨平阳霉素乳剂涂擦治疗增生期毛细血管瘤的微观机制,寻找促使血管瘤消退的新方法。方法选取6个月以内婴儿体表增生期毛细血管瘤60例。随机分为2组,每组30例。将平阳霉素制成乳剂,涂于用药组瘤体表面,1次/8 h;对照组则只涂以基质。用药7 d后两组均手术切除瘤体,制成石蜡切片观察细胞微观变化;采用TUNEL方法检测两组细胞凋亡指数,进行统计学处理;取两组各2例制成电镜标本,观察细胞超微结构变化。结果用药组较对照组凋亡细胞明显增多。对照组和用药组细胞凋亡指数分别为18.87±13.67,29.52±15.33,两者比较有非常显著性差异(t=2.842 P<0.01)。结论平阳霉素可诱导增生期毛细血管瘤细胞发生凋亡而促进其消退,其机制可能与阻断细胞周期和激活凋亡信号传导通路有关。Objective To explore the microcosmic mechanism of pingyangmycin treating infantile proliferating capillary hemangiomas as to find the optimal method for hemartgiomas＇ treatment. Methods Sixty samples of infantile proliferating hemangiomas were divided into control group （30 cases,aged from 2 days to 6 months） and experimental group（30 cases,aged from 2 to 6 months）. Pingyangmycin was made into emulsion and smeared on the surfaces of the leision in experimental group with 3 times every day as well as only matrix in control group. The specimens were resected on d7, then made into pathological slices and electron microscope slices in order to observe the cells microcosmic structure changes and uhrastructure changes. Furthermore, the apoptotic indexes of two groups were detected by the molecular biology method （TUNEL test）. Results The number of apoptotic cells were lower in control group（apoptotic index 18.87 ± 13.67）but higher apparently in experimental group（apoptotic index 29.52 ± 15.33）. The difference between two groups was significant （t = 2.842 P 〈0.01 ）. Conclusion Pingyangmycin can effectively accelerate apoptosis in infantile proliferating capillary hernangiomas whose mechanism is related to the blockage of cell cycle and activation of apoptotic signal transduction pathway.

关 键 词：平阳霉素 血管瘤 毛细管 细胞凋亡 

分 类 号：R732.2[医药卫生—肿瘤]