结直肠癌中抑癌基因ING1的表达研究  

Expression of ING1 and its protein p33/ING1 in colorectal carcinoma

作　　者：张森[1] 韦建宝[1] 周永淳[1] 陈利生[1] 

机构地区：[1]广西医科大学第一附属医院结直肠肛门外科,南宁530021

出　　处：《消化外科》2006年第5期336-339,共4页Journal of Digestive Surgery

基　　金：广西科学基金(桂科回0342018);教育部留学回国启动基金(No.教外司留2004-176)

摘　　要：目的探讨抑癌基因ING1及其蛋白p33/ING1在结直肠癌中的表达。方法用RT-PCR检测35例散发性结直肠癌中ING1mRNA的表达水平,并应用S-P法检测60例散发性结直肠癌及正常黏膜组织中p33/ING1蛋白的表达。结果结直肠癌组织、正常黏膜组织中p33/ING1蛋白阳性表达率分别为43.3%(26/60)、100%(60/60),两者比较差异有统计学意义(P<0.01)。p33/ING1在无淋巴结转移组及淋巴结转移组中的阳性表达率分别为57.6%(19/33)、25.9%(7/27),两者比较差异有统计学意义(P<0.05)。在DukesA、B期和DukesC、D期患者癌组织中p33/ING1的阳性表达率分别为56.7%(17/30)、30.0%(9/30),两者比较差异有统计学意义(P<0.05)。结论ING1及p33/ING1蛋白的低表达与散发性结直肠癌发生、发展密切相关,临床分期越差者表达水平越低。Objective To discuss the expression of tumor suppressor gene ING1 and its protein p33/ ING1 in development of sporadic colorectal carcinoma. Methods Reverse transcription-polymerase chain reaction was used to detect mRNA expression level of ING1 in 35 cases with sporadic colorectal carcinoma and streptomycete avidin-peroxidase immunohistochemistry to measure expression level of protein p33/ING1 in colorectal cancerous tissues and normal mucous tissues from 60 cases with sporadic colorectal carcinoma. Results Expression level of p33/ING1 mRNA in colorectal cancerous tissues was more significantly decreased than that in normal mucous tissues. Expression level of p33/ING1 mRNA in the tumors of patients at stage of Dukes A and B was significantly higher than that in patients at stage of Dukes C and D, as indicated that the expression decreased with poor clinical stage. Positive expression rates of p33/ING1 in colorectal cancerous tissues and normal mucous tissues were 43.3% （26/60） and 100% （60/60）, respectively, with significant statistical difference （ P〈0.01）. Positive expression rates of p33/ING1 in groups with or without lymph node metastasis were 25.9% （7/27） and 57.6% （19/33） respectively （ P 〈0.05）. Positive expression rates of p33/ING1 in patients at stage of Dukes A, B and at stage of Dukes C and D were 56.7% （17/30） and 30.0% （9/30） respectively （ P〈0.05）. Conclusions The decreased expression of p33/ING1 and low expression of protein p33/ING1 are closely related with development of sporadic colorectal carcinoma.

关 键 词：抑癌基因 ING1 结肠肿瘤 直肠肿瘤 

分 类 号：R735.3[医药卫生—肿瘤]