生化汤对血瘀证大鼠血管内皮细胞黏附分子表达的影响  

Effects of Shenghua Decoction on the expression of adhesion molecules in vascular endothelial cells of blood-stasis rats

作　　者：胡小勤[1] 陈利国[1] 屈援[2] 

机构地区：[1]暨南大学医学院 [2]暨南大学管理学院,广东广州510632

出　　处：《中成药》2006年第9期1330-1333,共4页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金资助项目(30070909)

摘　　要：目的:研究生化汤对血瘀证血管内皮细胞黏附分子表达的影响。方法:采用免疫组织化学和RT-PCR二种方法观察生化汤不同剂量(高、中、低)对血瘀证(大鼠)血管细胞黏附分子(VCAM-1)、血管内皮细胞胞间黏附分子-1(ICAM-1)、血小板-内皮细胞黏附分子(PECAM-1)和诱生型一氧化氮合酶(iNOS)表达的影响。结果:模型组VCAM-1、ICAM-1、PECAM-1、iNOS高表达,生化汤能减少造模动物VCAM-1、ICAM-1、PECAM-1、iNOS的表达,而且随着药物剂量的减少,各分子表达呈递增趋势,具有量效关系。结论:生化汤能降低血瘀证大鼠血管内皮细胞黏附分子表达,且量效关系明显。AIM： To study the effects of Shenghua Decoction on the expression of adhesion molecules in vascular endothelial cells （VECs） of blood-stasis rats. METHODS ： The expression of VCAM-1, ICAM-1, PECAM-1, iNOS and their corresponding mRNA in VECs were assayed by immunohistochemistry, image analysis and RT-PCR under the function of different Shenghua Decoction doses （ high, middle, low）. RESULTS ： Immunohistochemistry and image analysis indicated ： the expression of VCAM-1, ICAM-1, PECAM-1 and iNOS were higher in the model group than in the control group, Shenghua Decoction could decrease the expression of VCAM-1, ICAM-1, PECAM- 1 and iNOS in the model group, moreover as the dose decreased, the expression of these molecules increase, it was of dose-effect relation. CONCLUSION： Shenghua Decoction can downregulate the expression of adhesion molecules in vascular endothelial cells of blood-stasis rats, and the dose-effect relation is obvious.

关 键 词：血瘀证 内皮细胞 生化汤 黏附分子 免疫组织化学 RT-PCR 大鼠 

分 类 号：P066[天文地球]