半番鸭羽色性状AFLP标记初步研究  

Preliminary Study on AFLP Marker of Feather Color Trait in Mule Duck

作　　者：郑嫩珠[1] 陈晖[1] 缪中纬[1] 郑丽祯[1] 朱志明[1] 董晓宁[1] 

机构地区：[1]福建省农业科学院畜牧兽医研究所,福建福州350013

出　　处：《中国家禽》2006年第17期16-18,共3页China Poultry

基　　金：福建省科技厅重点项目(2005N023)。

摘　　要：本研究初步利用AFLP标记对半番鸭及其母本-北京鸭进行检测。结果显示,AFLP标记扩增带丰富,10对引物组合共检测到2046条扩增带,平均每对引物组合/每个池DNA可产生60.15条。半番鸭白羽性状特有的扩增带共有15条,分布在E+AAC/M+CTA、E+AAC/M+CTT、E+AAG/M+CAG、E+ACA/M+CTC、E+ACT/M+CTA和E+ACT/M+CTC等6对引物组合上,为半番鸭个体羽色与AFLP标记相关分析和验证提供了素材。In this paper,AFLP was used to detect feather color trait in mule duck and its female parent Beijing duck. The results indicated that there were rich bands amplified by AFLP marker. 2 046 bands were amplified by 10 pairs of primers. 60.15 bands on average were amplified with one pair or DNA pool. 15 specific bands were only found in white feather trait of mule buck,which lay in 6 pairs of E＋AAC/M＋CTA,E＋AAC/M＋CTF,E＋AAG/M＋CAG,E＋ACA/M＋ CTC,E＋ACT/M＋CTA and E＋ACT/M＋CTC,respectively. All these results lay the foundation for the further evaluation and analysis on the relationship between AFLP marker and feather color trait in mule duck individually.

关 键 词：半番鸭 北京鸭 羽色 AFLP标记 

分 类 号：S661.1[农业科学—果树学]