肝组织中糜酶浓度在慢性肝炎肝纤维化中的意义  

Hepatic chymase content in relationship between chronic hepatitis and fibrosis

作　　者：尹明实 朴红心[1] 周振霞[2] 韩学吉[1] 太永日[1] 

机构地区：[1]延边大学医学院附属医院感染症科,延吉133000 [2]安图县医院,安图133600

出　　处：《中国免疫学杂志》2006年第9期876-877,共2页Chinese Journal of Immunology

摘　　要：目的:为探讨肝组织中糜酶(Chymase)浓度在慢性病毒性肝炎肝纤维化中的意义。方法:采用酶联免疫吸附实验(ELISA)检测45例慢性肝炎肝组织中Chymase浓度,观察不同的纤维化分期(S1-S4),其肝组织中Chymase浓度;用免疫组织化学染色法观察Chymase单克隆抗体标记的肥大细胞分布情况。结果:慢性肝炎纤维化分期重的S3和S4患者,其肝组织中Chymase浓度(S3+S4=31.3±24.6ng/mg)明显高于纤维化轻的S1和S2患者(S1+S2=5.7±4.8ng/mg),P<0.01;免疫组化染色结果,Chymase标记的肥大细胞主要分布于纤维化旺盛的汇管区与类洞壁,其分布与纤维化部位相一致,且肝组织中Chymase浓度高的患者,其肝组织内Chymase标记的肥大细胞分布增多。结论:肝组织中Chymase浓度可能与慢性肝炎肝纤维化关系密切。Objective：To investigate spatial relationship between human chymase and Fibrosis in liver specimens affected by chronic hepatitis. Methods ：The chymase levels were determined by enzyme-linked immunosorbant assay（ELISA） in 45 cases of chronic hepatitis. Mast cell distribution in the specimens was determined immunohistochemically using anti-chymase antibudy. Results：Hepatic chymase concentrations in S3 and S4 cases were greater than in S1 and S2 （ S3 ＋ S4 = 31.3 ＋ 24. 6 ng/mg vs S1 ＋ S2 = 5.7 ＋ 4. 8 ng/mg; P 〈 0.01 ）. Cells immunoreacfive for chymase were seen throughout portal areas and intralobular sinusoidal walls, largely colocalizing with fibrosis. Conclusion：Chymase appears to be involved in hepatic fibrosis in chronic hepatitis.

关 键 词：糜酶 慢性肝炎 肥大细胞 肝纤维化 血管紧张素 

分 类 号：R[医药卫生]