甲状腺功能低下大鼠肝、肾α-磷酸甘油脱氢酶和过氧化物酶活性的变化  

Activity Observation of Mitochondria α-Dlycerophosphate Dehydrogenase in Liver and Kidney and Thyroid Peroxidase in the Experimental Rats at Hypothyroidism

作　　者：刘桂云[1] 刘超[2] 高淑贤[2] 

机构地区：[1]锦州医学院中心实验室,锦州121001 [2]锦州医学院生物化学教研室

出　　处：《数理医药学杂志》2006年第5期541-542,共2页Journal of Mathematical Medicine

摘　　要：目的:观察甲状腺功能低下(甲低功)时大鼠肝、肾线粒体中α-磷酸甘油脱氢酶(α-GPD)和甲状腺过氧化物酶(TPO)活性变化。方法:用0.5%NaClO4饮水复制实验大鼠甲低功模型,分实验组(NaClO4)和对照组(自来水)。肝、肾线粒体制备,按本实验室改进的Schneider氏法;α-GPD测定采用Lee法;TPO采用Hosoya氏法。结果:实验组肝、肾α-GPD活性明显低于对照组(P<0.01),实验组TPO活性明显高于对照组(P<0.01),甲状腺含碘量及血清T4含量明显低于对照组(P<0.01),TSH水平高于对照组(P<0.01)。结论:实验大鼠肝、肾α-GPD酶活性明显下降,而甲状腺TPO活性明显增高,说明在甲低功早期,血清T4含量下降,通过负反馈作用使垂体TSH分泌增高,促进甲状腺TPO的合成和甲状腺滤泡的增殖。Objective. To observe the changes of mitochondrial α-glycerophosphate dehydrogenase activity in liver and kidney and thyroid peroxidase activity of the experimental hypothyroidism rats. Methods. The experimental hypothyroidism rats were duplicated with 0.5% sodium perchlorate, with water as control. We made mitochondria of liver and kidney as proved Schneider＇s,α-GPD as Lee＇s and TPO as Hosoya＇s. Results： Hepatic and kidney＇s α-GPD activity of experiment and the iodine levels of thyroid and serum T4 were lower than that of the control （P〈0.01）, while TPO activity and TSH of experiment were higher than that of the control （P〈0.01）. Conclusions： The activity of α-GPD in liver and kidney of experimental hypothyroidism rats were lowered obviously. But TPO activity of experimental group was higher than that of the control （P〈0.01）. A negative relationship between serum T4 and TPO activity was established in the early hypothyroidism.

关 键 词：甲状腺功能 Α-磷酸甘油脱氢酶 过氧化物酶 

分 类 号：R-332[医药卫生]