莲心碱对氧自由基引发红细胞膜损伤的保护作用  

Protective effect of Liensinine on oxidative damage of erythrocyte ghost by oxygen free radicals

作　　者：王辉[1] 周立[1] 刘刚[1] 

机构地区：[1]武汉大学人民医院药学部,湖北武汉430060

出　　处：《中国药理学通报》2006年第9期1088-1091,共4页Chinese Pharmacological Bulletin

基　　金：湖北省科技攻关资助项目(No2002AA302B04)

摘　　要：目的研究莲心碱对超氧阴离子自由基(O2.)引发的红细胞膜氧化损伤的影响。方法采用邻苯三酚自氧化产生的超氧阴离子自由基O2.致红细胞膜损伤,以此为膜过氧化损伤实验模型,以脂质过氧化产物丙二醛(MDA)、细胞膜封闭能力、细胞膜自氧化速率、膜脂流动性(MFU)为指标,观察莲心碱对红细胞膜氧化损伤的保护作用。结果O2.能引起红细胞膜脂质过氧化,丙二醛(MDA)含量、自氧化速率增加、膜流动性以及膜封闭度下降。而红细胞膜经过一定量的莲心碱(80,100,120mg.L-1)预先处理后,膜MDA含量明显减少,自氧化速率明显降低,膜流动性和膜封闭度提高。结论莲心碱对氧自由基引发的红细胞膜损伤具有一定的保护作用。Aim To study the influence of Liensinine （LIE） on the oxidative damage of erythrocyte ghost. Method The erythrocyte ghost oxidative injury caused by super oxide anion （O2^-） from the auto-oxidation of pyrogallic acid was used as model. The effect of LIE on oxidated erythrocyte ghost was observed by the measurement of the malonidial aldehyde （MDA）-the degradation product of lipid peroxidation, blocking degree, speed of auto-oxidation and membrane fluidity. Result O2^- caused lipid peroxidation of erythrocyte ghost, increased the content of MDA and the speed of auto-oxidation, decreased membrane fluidity, and alleviated blocking degree. But LIE（80, 100, 120 mg·L^-1） could decrease the content of MDA and speed of auto-oxidation markedly, improve the membrane fluidity and blocking degree significantly, and inhibit the lipid peroxidation. Conclusion LIE has protective action on the oxidative damage of erythrocyte ghost initiated by O2^-.

关 键 词：莲心碱 氧自由基 红细胞膜 邻苯三酚 过氧化损伤 

分 类 号：R-332[医药卫生] R282.71