改良大鼠蛛网膜下腔出血模型  

Modified rat model of subarachnoid hemorrhage

作　　者：朱庆[1] 刘艳霞[1] 李刚[1] 蔡敏[1] 王敏伟[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《中国药理学通报》2006年第9期1144-1146,共3页Chinese Pharmacological Bulletin

摘　　要：目的改良大鼠蛛网膜下腔出血(SAH)模型。方法应用自体动脉血直接注入蛛网膜下腔的方法复制SAH模型,但不暴露环枕膜,在顶枕骨交界线中点钻一骨孔,插管8mm至小脑延髓池注血造模。建立模型后测定大鼠血液流变学指标,用DAB染色法显示大鼠海马CA1区微血管,用Image-ProPlus图像分析系统测定微血管直径、密度(MVD)、微血管面积比(MVA)。结果SAH3d后模型组大鼠全血粘度(ηb)、红细胞聚集指数(AL)明显升高,海马CA1区MVD、MVA明显下降。结论改良的大鼠蛛网膜下腔出血模型方法稳定可靠,手术过程简单,容易操作。Aim To develop a modified model of subarachnoid hemorrhage in rats. notheds The SAH model was induced by injecting autologous blood into the cisterna magna without exposing the occipitoaxial ligament. The main modified point was a hole bored by a dental drill at the middle point of the boundary between the parietal bone and the occipital bone, then a catheter was inserted 8 mm to cisterna magna and the autologous blood was injected. Hemorrheological parameters were measured after the model was created. The intrinsic oversxidase method was used to show the microvessel of CA1 region. The diameter, density, and the aera of microvessel were measured and ananlyzed by Image-Pro Plus image analysis system. Results The total blood viscosity （ηb） and erythroagglutation index （AL） in SAH model group increased significantly, however, the microvesse area and microvesse density in SAH model group decreased obviously. Conclusion The modified model of subarachnoid hemorrhage in rats is reliable and easy to operate on.

关 键 词：蛛网膜下腔出血 血液流变学 内源性过氧化酶 微血管图像分析 

分 类 号：R-332[医药卫生] R363-332