油炸食品中两种酚类抗氧剂的反相高效液相色谱法测定  

DETERMINATION OF TWO PHENOLIC ANTIOXIDANTS IN FRIED FOODS BY THE REVERSED-PHASE HPLC

作　　者：周建科[1] 张前莉[1] 韩康[1] 张立[1] 

机构地区：[1]河北大学理化分析中心河北省分析科学技术重点实验室,河北保定071002

出　　处：《食品研究与开发》2006年第7期166-168,共3页Food Research and Development

摘　　要：采用反相高效液相色谱法测定油炸食品中叔丁基对苯二酚(TBHQ)、抗氧剂2246的含量。正己烷饱和的乙腈做提取剂,以甲醇为流动相,227nm波长检测。在0.11mg/L～56.00mg/L范围内,线性相关系数均为0.9999,检出限分别为:0.22mg/L,0.11mg/L,相对标准偏差在0.19%～2.87%之间,平均回收率均在85.0%以上。3种油炸方便面、薯条、豌豆脆样品中未检出抗氧剂2246,均有叔丁基对苯二酚检出。A reversed-phase high performance liquid chromatography method was developed for the simultaneous quantitative determination of tea-Butyl hydroquinone（TBHQ） and 2,2＇-methyllenebis （4-methyl-6-tertiarybutyl phenol） in fried foods. The TBHQ were extracted from the samples by acetonotrile that saturated by n-hexane. Methanol was used as the mobile phase and the detection wavelength was 227 nm.The calibration curve was linear between 0. 11 mg/L to 56.00 mg/L, and the linear relativity were both 0.999 9.The limits of the detection were 0.22 mg/L and 0.11 mg/L. The average spiked recoveries were all above 85.0 %, and the relative standard deviations were between 0.19 % and 2.87 %.The results indicated that tea-Butyl hydroquinone was discovered in these samples ,but 2,2＇-methyllenebis （4-methyl-6-tertiary-butyl phenol） was not found out.

关 键 词：反相高效液相色谱 叔丁基对苯二酚 抗氧剂2246 

分 类 号：TS207[轻工技术与工程—食品科学]