海水浸泡对失血性休克并腹部开放伤实验大鼠肿瘤坏死因子α及肠道热休克蛋白HSP-70的影响  

Influence of Sea Water Immersion on TN-α and Enteric HSP-70 of Experimental Rats with Hemorrhagic Shock and Open Abdominal Injury

作　　者：陈战[1] 应可满[1] 刘哓娟[1] 夏挺[1] 

机构地区：[1]解放军第174医院普外,福建厦门361003

出　　处：《临床军医杂志》2006年第4期397-399,共3页Clinical Journal of Medical Officers

摘　　要：目的探讨海水浸泡失血性休克并腹部开放伤对实验大鼠血浆肿瘤坏死因子α(TNFα-)和肠道热休克蛋白HSP-70水平变化的影响。方法建立失血并腹腔海水浸泡伤动物模型。实验大鼠随机分为对照组(n=6)、失血创伤组(n=14)、失血创伤海水浸泡组(n=14),失血创伤组为单纯失血性休克并腹部开放伤,失血创伤海水浸泡组将动物致伤后置入人工配置的海水中,于伤前和浸泡后30m in及1,1.5,3 h取血测定TNFα的变化,取肠道组织测定HSP-70的表达。结果失血创伤海水浸泡组血中TNFα较失血创伤组伤后明显升高并且高峰出现时间明显提前,肠道损伤病理变化明显重于失血创伤组,肠道组织中HSP-70含量在创伤早期显著升高,后又明显降低。结论TNFα-的过度表达在失血性休克并腹部开放伤后海水浸泡的病理生理变化中起重要作用,肠道组织细胞中HSP-70的异常表达在肠道组织细胞的应激反应机制中,可能有重要的意义。Objective To investigate the influence of sea water immersion on tumor necrosis factor-α （TNF-α） and enteric hot shock protein-70 （HSP-70） in experimental rats with hemorrhagic shock and open abdominal injury. Methods Animal model rats of hemorrhage and open abdominal injury, with or without sea water immersion into abdominal cavity, were set up. Experimental rats were divided into three groups, i.e. normal control group （Group 1, n = 6 ）, hemorrhagic shock group （Group 2, n = 14） and hemorrhagic shock ＋ sea water immersion group （ Group 3, n = 14）. Group 2 consisted of the rats with hemorrhagic shock group plus open abdominal injury, and Group 3 includedthe rats with additional sea water immersion. Results Not only the TNF-cdevel of Group 3 in blood inreased more significantly than that of Group 2, but its peak appeared earlier as well. Furthermore, the pathological change of Group 3 was severer than that of group 2, and its enteric HSP-70 level decreased obviously along with the duration of sea water immersion, in spite of a short rise at the early stage of trauma. Conclusion The over-expression of TNF-α takes an important role in the pathological change of sea water immersion on the basis of hemorrhagic shock and open abdominal injury, and the abnormal expression of HSP-70 in enteric tissue may exert an influence on the stress reaction of enteric ceils.

关 键 词：腹部外伤 失血性休克 海水浸泡 肿瘤坏死因子 热休克蛋白 

分 类 号：R363.16[医药卫生—病理学] R656[医药卫生—基础医学]