棕榈氯霉素卵磷脂O/W型微乳制剂的研究  

Research of the Chloramphenicol Palmitate In Phosphatidylcholine O/W Microemulsions

作　　者：王刚[1] 尤静 王玉东[1] 王九成[3] 傅经国[4] 赵清香[1] 刘民英[1] 

机构地区：[1]郑州大学材料工程学院,郑州450052 [2]陕西医学高等专科学校,西安710068 [3]河南科学院化学研究所,郑州450002 [4]西安力邦医药科技有限责任公司,西安710075

出　　处：《江苏药学与临床研究》2006年第4期223-225,共3页Jiangsu Pharmacertical and Clinical Research

摘　　要：目的棕榈氯霉素卵磷脂O/W型微乳制剂的制备与含量检测。方法用十六酸异丙酯溶解棕榈氯霉素作油相,卵磷脂和吐温80混合作为表面活性剂,乙醇为助表面活性剂,制备O/W型微乳制剂;用高效液相色谱法进行棕榈氯霉素含量的测定。色谱条件:C18柱(250 mm×4.60 mm,5μm),流动相为甲醇液,柱温25℃,流量为1.0 ml.min-1,检测波长为271 nm,进样体积20μl。结果取得了较好的棕榈氯霉素卵磷脂O/W型微乳制剂及较好的含量检测方法。讨论棕榈氯霉素可以制成卵磷脂O/W型微乳制剂,用高效液相色谱法进行含量测定。AIM To prepare O/W microemttlsions of chloramphenicol palmitate in phosphatidylcholine. METHODS Using dissolved chloramphenicol palmitate in isopropyl palmitates as oil phase, lecithin and tween-80 as suffactant and alcohol as eosuffaetant to prepare O/W mieroemulsion. HPLC was used to determine chloramphenieol pahnitate on C18 column（250 mm × 4.60 mm, 5μm）, the mobile phase consisted of methanol,the flow rate was 1.0 ml·min^-1 ,the column temperature of 25 ℃ ,the injection volume was 20 μl. RESULTS Chloramphenieol palmitate in phosphatidyleholine O/W mieroemttlsions were prepared and the method of determination was developed, CONCLUSION Chloramphenicol palmitate can be used in O/W phosphatidyeholine mieroemulsions, HPLC can be used to determine.

关 键 词：微乳 O/W型 卵磷脂 棕榈氯霉素 高效液相色谱法 

分 类 号：R944.1[医药卫生—药剂学]