特大剂量γ射线照射的染色体畸变剂量效应曲线  

Establishment and verification of dose-response curve of chromosomal aberrations after exposure to very high dose γ-ray

作　　者：陈英[1] 骆亿生[1] 曹珍山[1] 刘秀林[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《辐射研究与辐射工艺学报》2006年第4期241-244,共4页Journal of Radiation Research and Radiation Processing

基　　金："十五"指令性课题(01L019)资助

摘　　要：为准确估算大剂量事故受照者的生物剂量,拟合6—22Gy照射后染色体畸变剂量效应曲线。采用60Coγ射线照射离体人血,浓集有核细胞,一步法培养52h、68h和72h收获制片。计数双(多)着丝点+环数目,比较不同培养时间畸变差异,拟合剂量效应曲线及数学方程。用大剂量事故受照者的剂量结果对曲线进行验证。实验表明,大剂量照射后需适当延长细胞培养时间,52—72h获得的染色体双+环畸变率无明显差异。新拟合的剂量效应曲线符合线性平方模型Y=-2.269+0.776D-7.868×10-3D2。经事故剂量验证结果可靠。本研究首次建立了6—22Gy照射后染色体双+环剂量效应曲线及数学方程,为特大剂量照射后的准确剂量估算提供了可依据的方法。To estimate accurately biological dose of the victims exposed to high dose, the dose-response curves of chromosome aberration induced by 6-22Gy 60^Co γ-ray were established. Human peripheral blood in vitro was irradiated, then lymphocytes were concentrated, cultured 52h, 68h and 72h and harvested. The frequencies of dicentrics （multi-centrics） and rings were counted and compared between different culture times The dose-response curves and equations were established, as well as verified with high dose exposure accidents. The experiment showed that the culture time should be prolonged properly after high dose exposure, and no significant differences were observed between 52-72h culture. The dose-response curve of 6-22Gy fitted to linear-square model Y=2.269＋0.776D-7.868 ×10^-3D^2 and is reliable through verification of the accident dose estimations. In this study, the dose-response curve and equation of chromosome dic＋r after 6-22Gy high dose irradiation were established firstly, and exact dose estimation can be achieved according to it.

关 键 词：特大剂量γ射线 染色体畸变 剂量效应曲线 事故剂量估算 

分 类 号：Q691[生物学—生物物理学]