百日草链格孢菌粗毒素的生产、提取及稳定性的研究  

Production,Extraction and Stability for Crude Phytotoxin Produced by Alternaria zinniae

作　　者：李荣金[1] 强胜[1] 

机构地区：[1]南京农业大学杂草研究室,南京210095

出　　处：《中国生物工程杂志》2006年第8期67-71,共5页China Biotechnology

基　　金：国家"863"计划资助项目(2001AA246012)

摘　　要：百日草链格孢菌是从世界性恶性杂草苍耳罹病植株上分离得到的植物病原真菌，研究表明该菌对紫茎泽兰有致病作用，可产生致病的次生代谢产物-毒素。对该菌的次生代谢产毒能力情况进行了研究。对培养基条件进行了筛选，确定了有利于该菌产毒的温度为25℃、pH值为6．5、培养时间为9-12d、转速110r／min、黑暗与充分溶氧等培养条件。利用大规模发酵与发酵产物后提取工艺获取粗毒素，并通过分析贮存时间、温度、光照对粗毒素稳定性的影响，表明其具有开发为天然微生物源除草剂的潜力。Alternaria zinniae was a plant fungal pathogen isolated from a world-wide weed Xanthium occidentale, which could cause some weeds of Asteraceae disease. It was found that the fungus made disease spot on the leaf through producing secondary metabolite-phytotoxin. The toxin-producing capability of the fungus was studied. The optimal cultural conditions for producing phytotoxin were temperature 25℃, pH 6.5, cultured period 9 - 11d, rotating speed 110r/min, darkness and enough dissolved 02, crude toxin was obtained through large scale fermentation. Analysis on the influence of time, temperature, lightness for storing on the stability of phytotoxin of Alternaria zinniae showed that the phytotoxin had the potential to develop as a herbicide originating from microorganism.

关 键 词：百日草链格孢菌 产毒 毒素提取 大孔吸附树脂 稳定性 

分 类 号：Q819[生物学—生物工程]