低分子肝素对人肝癌裸鼠模型中COX-2表达影响的研究  

Low molecular weight heparins inhibit cyclooxygenase-2 expression in human hepatocarcinoma implanted in nude mice

作　　者：袁克莉[1] 汪昱[1] 郑起[1] 闫钧[1] 

机构地区：[1]上海交通大学附属第六人民医院,上海200233

出　　处：《山东医药》2006年第26期14-16,共3页Shandong Medical Journal

基　　金：上海市卫生局资助项目(00441)

摘　　要：目的研究低分子肝素对人肝癌裸鼠模型(LC I-D 20)中环氧合酶-2(COX-2)表达的影响。方法采用人肝癌组织移植制作人肝癌裸鼠模型36只并随机分为对照组、化疗组、肝素组和联合组各9只,造模第1天:对照组腹腔注射生理盐水;化疗组腹腔注射5-氟脲嘧啶(5-Fu)+顺铂(DDP);肝素组皮下注射低分子肝素;联合组腹腔注射5-Fu+DDP、皮下注射法安明,1次/d,均共用5周。观察各组原位肿瘤体积、抑瘤率、肿瘤微血管密度(M VD)、血管内皮生长因子(VEGF)、COX-2及血清AFP水平。结果化疗组、肝素组和联合组裸鼠原位肝癌体积明显小于对照组;化疗组与对照组相比M VD差异无显著意义,而肝素组与联合组的M VD、COX-2及VEGF阳性率、血清AFP均明显低于化疗组和对照组(P<0.05);肝素组和联合组抑瘤率低于化疗组,但差异无统计学意义。结论低分子肝素对人肝癌裸鼠模型中肿瘤血管形成具有抑制作用,该作用与抑制COX-2表达有关。[Objective] To study effects of low molecular weight heparins on cyclooxygenase-2 （COX-2） of hepatocarcinoma in vivo. [Methods] Metastatic model simulating human hepatoearcinoma was established by orthotopic implantation of human tumor tissue into nude mice. Then were randomly submitted into different treatment groups ： control group with saline solution, chemotherapy group with 5-Fu and CDDP i. p. , Fragmin group （subcutaneous injection） ,combination group. After 7 weeks of treatment, tumor volume, inhibitive rates , intratumoral microvessel density （MVD）, vascular endothelial growth factor （VEGF）, COX-2 and AFP. [Results ] Compared with control group, implanted tumors were significantly reduced in weight in chemotherapy, Fragmin and combination groups with corresponding inhibition rates 91. 63%, 69. 40% and 71.10% respectively. MVD decreased significantly in Fragmin and combination groups（4.83±1.83 and 6. 50±2.35）,and there was no significant difference between chemotherapy and control group. VEGF,COX-2 staining and AFP level were significantly reduced in cancer tissue in Fragmin group and combined treatment group compared with the chemotherapy and control group. The growth of human hepatocarcinoma implanted in nude mice was inhibited in Fragmin group and combined treatment group compared with chemotherapy, but the difference had no statistical significance. [Conclusion] Low molecular weight heparins （Fragmin） can inhibit growth hepatocarcinoma, possibly through inhibiting tumor angiogenesis. And Low molecular weight heparins maybe inhibit tumor angiogenesis by inhibiting COX-2 expression.

关 键 词：肝肿瘤 肝素 环氧合酶-2 

分 类 号：R735.5[医药卫生—肿瘤] R735.7[医药卫生—临床医学]