cDNA末端扩增技术的研究进展  

Research progress of rapid amplification of cDNA ends

作　　者：郝敏[1] 谷守芹[2] 韩建民[1] 董金皋[1] 

机构地区：[1]河北农业大学真菌毒素实验室,河北保定071001 [2]保定师范专科学校生物系,河北保定071051

出　　处：《河北林果研究》2006年第2期157-161,共5页Hebei Journal of Forestry and Orchard Research

摘　　要：cDNA末端扩增技术是一种以PCR反应为基础,快速获得已知cDNA序列3′端和5′端的方法,是克隆全长cDNA序列的主要手段之一。本文从RACE原理出发,对RACE技术3个关键步骤(反转录、TdT加尾、PCR扩增)的优化和改良措施进行了透彻分析,并详细阐述RACE的最新研究进展———“电子”cDNA克隆。Rapid amplification of cDNA ends is a method obtaining 3＇ ends and 5＇ ends of known cDNA fragments based on PCR reaction, and one of the primary means cloning cDNA fragments. Beginning with the principle this article analyses the three critical steps （transcription, TdT tailing, PCR amplification） of RACE technique in detail, and expatiates the latest RACE techniques （Marathon - PCR, SMART - RACE, In silico cloning）.

关 键 词：RACE 全长CDNA序列 PCR 反转录 

分 类 号：S791.117.05[农业科学—林木遗传育种]