PBMCs中HBV逆转录酶P基因的mRNA表达X基因的整合与肝细胞癌变的关系  

THE mRNA EXPRESSION OF GENE P IN HBV REVERSE TRANSCIPTASE OF PBMCs,THE INTEGRATION OF GENE X AND THEIR RELATIONS TO PRIMARY HEPATOMA

作　　者：王淑霞[1] 王力秋[1] 刘化勇[1] 赵海燕[1] 

机构地区：[1]临沂市人民医院放疗中心,山东临沂276003

出　　处：《山东医学高等专科学校学报》2006年第1期33-35,共3页Journal of Shandong Medical College

基　　金：临沂市科技发展计划项目(No.0508030)

摘　　要：目的在外周血单个核细胞(PBMCs)中研究乙型肝炎病毒(HBV)DNA多聚酶/逆转录酶P基因的mRNA表达、X基因的整合与肝细胞癌变的关系。方法设计HBV P基因引物P1、P2,及X基因引物X1、X2。分离HBsAg阳性40例原发性肝癌病人及36例慢性乙肝病人PBMCs,分别提取其基因组总DNA和总RNA,RNA逆转录合成cDNA;PCR检测HBV的X基因整合;RT-PCR方法检测HBV的DNA多聚酶/逆转录酶P基因在PBMCs中的mRNA表达。结果PBMCs中X基因片段的整合率在HCC组为79.5%(31/40),与CH组的整合率50%(18/36)无统计学差异(P>0.05)。P基因的mRNA表达率HCC组为10%(4/40),与CH组表达率33.3%(12/36)有统计学差异(P<0.05)。结论在HCC阶段HBV病毒复制相对静止,大量X基因整合最终导致HCC的发生。Objective To explore the expression of mRNA of gen P in HBV polymerase/reverse transcriptase of peripheral blood mononuclear cells （ PBMCs）, the integration of gene X and their relations to hepatoma. Methods Primers P1 and P2 were designed for HBV gene P, and Xl and X2 for genex. PMBCs were isolated from 40 HBsAg-positive cases of primary hepatoma and 36 eases of chronic hepatitis B（HB）. Total DNA and total RNA in genome were extracted. RNA was made into cDNA after reverse synthesis. The integrations of HBV gene X were detected through PCR. mRNA expressions of gene P in PBMCs in HBV were detected through RT-PCR method. Results The integration rate of gene X in PBMCs of the primary hepatoma （PH） group and the chronic HV（CHV） group were 79.5 % （31/40） and 50 % （18/36） respectively （ P 〈 0.05 ）. The mRNA expression rates of gene P in the former group and the latter group were 10% （4/40） and 33.3% （12/36） respectively （ P 〈0. 05）. Conclusion HBV replications remain relatively resting in the primary hepatoma group, and the intergration of a great number of gene X leads to the occurrence of the disease.

关 键 词：原发性肝细胞癌 乙型肝炎病毒X P基因 外周血单个核细胞 整合 聚合酶链反应 

分 类 号：R735.7[医药卫生—肿瘤]