TCT查验子宫颈涂片中HPV感染的研究  

Detection of HPV infection in cervical Pap smears by TCT test

作　　者：洪慧丽 涂知明[2] 

机构地区：[1]武汉市第三医院妇产科,430060 [2]华中科技大学生命科学与技术学院,430074

出　　处：《中国优生与遗传杂志》2006年第9期79-80,107,共3页Chinese Journal of Birth Health & Heredity

摘　　要：目的评价液基薄层细胞检测技术(TCT)检测宫颈涂片中人乳头状瘤病毒(human papilloma virus,HPV)感染的敏感性与准确性。方法对1030例外阴尖锐湿疣患者的子宫颈涂片,采用TCT检测技术进行细胞病理学诊断,其诊断标准依据The Bethesda System(TBS)分类法。同期行阴道镜指引下的子宫颈活组织检查和聚合酶链反应(PCR)技术检测宫颈拭子中的HPVDNA。结果应用PCR检测1030例宫颈拭子HPVDNA,阳性者37例(35.92%)。宫颈活组织学检查结果为ASCUS57例(5.53%);上皮内病变CIN11例,占总数的1%;占HPV(+)百分率为29.73;占57例为非典型麟状细胞阳性的19.3。结论TCT作为一种细胞病理学检测技术,用于诊断宫颈HPV感染或亚临床感染,有临床应用价值。Objective： The sensitivity and accuracy of the Thinprep pap test （TCT） in diagnosis of human papillomavirus （HPV） infection in cervical Pap smear were evaluated. Methods： Cervical Pap smears obtained from 1030 patients with vulva condyloma acuminata were examined by TCT, The diagnostic criteria were based on The Bethesda System （TBS）. Simultaneously HPV DNA in cervical mucus was examined by polymerase chain reaction （PCR） technique and cervical biopsy taken under the guidance of colposcopy for pathological examination. Results： Use PCR to detect the HPV DNA in 1030 samples, 37 （35.92%） samples are HPV DNA posifive; TCT test 57 （5.53%） are ASCUS positive; There are 11 CIN （1%） happened, account for 29, 73% of HPV （ ＋ ） samples, and account for 19. 3% of ASCUS positive samples. Conclusion： The results indicates that TCT, a cytologic pathological technique, is a useful method in diagnosis of clinical and subclinical cervical HPV infection.

关 键 词：乳头状瘤病毒(HPV) 宫颈涂片 液基薄层细胞检测技术(TCT) 

分 类 号：R737.33[医药卫生—肿瘤]