血管内皮细胞生长因子诱导大肠癌LOVO细胞粘附作用  

The adhesion effect of large intestine cancer cells induced by vascular endothelial growth factor

作　　者：毛华[1] 宋卫生[1] 黄纯帜[1] 

机构地区：[1]南方医科大学珠江医院消化内科,广州510280

出　　处：《全科医学临床与教育》2006年第5期373-375,共3页Clinical Education of General Practice

摘　　要：目的研究观察血管内皮细胞生长因子(vascular endothelial growthfactor,VEGF)对大肠癌LOVO细胞粘附作用。方法采用3H-TdR掺入及鼠尾胶粘附实验测定VEGF诱导大肠癌LOVO细胞同质性和异质性粘附作用。结果1ng/ml、5ng/ml VEGF诱导LOVO细胞60min、90min3H-TdR掺入实验(dpm/min)分别为1558.27±251.11、1380.03±198.05和2829.30±2262.14、2641.6±473.24,10ng/ml VEGF诱导LOVO细胞60min、90min、120min3H-TdR掺入实验分别为1302.20±201.04、2797.50±413.24、2066.79±187.97,显著低于对照组60、90、120min的1984.14±216.74、3361.00±175.27、4173.40±373.98,P<0.05或0.01;5ng/ml、10ng/ml VEGF诱导LOVO细胞60分钟鼠尾胶粘附实验OD值为0.250±0.018、0.230±0.036,1ng/ml、5ng/ml、10ng/ml VEGF诱导LOVO细胞90min、120min鼠尾胶粘附实验OD值分别为0.263±0.021、0.373±0.083、0.378±0.080和0.329±0.056、0.389±0.095、0.419±0.102,分别高于对照组的0.130±0.025、0.143±0.036、0.210±0.028,P<0.05或0.01。结论VEGF可以促进大肠癌细胞异质性粘附作用以及降低大肠癌细胞的同质性粘附作用。Objective To observe the adhesion effect of large intestine cancer cell LOVO induced by vascular endothelial growth factor （VEGF）. Methods The function of homotypic adhesion and hetertypic adhesion in large intestine cancer cell LOVO was measured with 3H-TdR infiltration and experiment of rat tail colloid . Results After 60 and 90 minutes, the value （dpm/min） of 3H-TdR infiltration in LOVO ceils induced by lng/ml, 5ng/ml VEGF were1558.27 ± 251.11, 1380.03 ± 198.05, and 2829.30± 2262.14, 2641.60± 473.24, that by 10ng/ ml VEGF were 1302.20±201.04,2797.50 ± 413.24,and 2066.79 ± 187.97, which was dramatically lower than that in control group , P 〈 0.05 or 0.01 ; After 90 and 120 minutes, the OD value of experiment of rat tail colloid in LOVO ceils induced by 1ng/ml, 5ng/ml, 10ng/ml VEGF were 0. 263 ± 0. 021, 0. 373 ± 0. 083, 0. 378 ± 0. 080 and 0. 329 ± 0. 056, 0. 389 ±0. 095, 0.419 ± 0. 102, respectively, which was remarkably higher than that in control group 0. 130 ± 0. 025, 0. 143 ± 0. 036, 0.210 ± 0.028, P 〈 0.05 or 0.01. Conclusion VEGF can promote hetertypic adhesion and decrease homotypic adhesion of large intestine cancer cell LOVO.

关 键 词：血管内皮细胞生长因子 大肠癌细胞株 粘附 转移 

分 类 号：R735.34[医药卫生—肿瘤]