细胞凋亡显像剂^(99)Tc^m-HYNIC-Annexin Ⅴ的制备及其生物评价  

Preparation and Evaluation of ^(99)Tc^m-HYNICAnnexin Ⅴ for Apoptosis Imaging

作　　者：祁本忠[1] 陈大明[1] 杨红伟[1] 张文辉[1] 窦勤毅[1] 袁荣玺[1] 刘萌[2] 王荣福[2] 张春丽[2] 

机构地区：[1]中国原子能科学研究院同位素研究所,北京102413 [2]北京大学第一医院核医学科,北京100034

出　　处：《同位素》2006年第3期140-145,共6页Journal of Isotopes

摘　　要：合成了双功能螯合剂琥珀酰亚胺-6-肼基吡啶-3-甲酸(HYNIC)。并将HYNIC成功地应用于AnnexinⅤ的偶连和标记中。结果表明:每个AnnexinⅤ的蛋白分子能偶连2个HYNIC,标记率为98%,标记物的放化纯度在6 h后仍然>95%;99Tcm-HYNIC-AnnexinⅤ在动物模型中能有效检测细胞凋亡。因此,99Tcm-HYNIC-AnnexinⅤ有望成为检测肿瘤治疗效果的显像剂。Bifunctional chelating agent succinimidyl 6 tert-boc-hydrazinopyridine-3-carboxylic acid（HYNIC） is synthesized and successfully applied in the conjugating and radiolabeling of annexin V. One molecular of annexin V is conjugated with two molecular of HYNIC. The labeling ratio of the labeled compound is 98~, and the radiochemical purity of 95% is remained after 6 h. ^99Tc^m-HYNIC Annexin V has heen successfully evaluated in animal models and the results show that the cell apoptosis can be assaied by which.

关 键 词：^99TC^M HYNIC ANNEXIN V 细胞凋亡 显像剂 

分 类 号：TQ463.7[化学工程—制药化工] R817[医药卫生—影像医学与核医学]