SPME/GC/MS法分析白车轴草花挥发性化学成分  

Analysis of chemical constituents of the essential oil fromflowers of Trifolium repensL.by SPME-GC-MS

作　　者：吴彩霞[1] 王倩嵘[2] 罗君[2] 康文艺[1] 

机构地区：[1]河南大学天然药物研究所,河南开封475004 [2]河南省疾病预防控制中心,河南郑州45003

出　　处：《河南大学学报（医学版）》2006年第3期17-20,共4页Journal of Henan University:Medical Science

基　　金：国家自然科学基金项目(30500646);中国博士后科学基金项目(2004035254)

摘　　要：目的:分析白车轴草花的挥发性化学成分。方法:采用固相微萃取/气相色谱/质谱(SPME/GC/MS)联用技术。结果:共分离出76个化学成分,鉴定出其中的36个成分,并用峰面积归一化法测定其相对百分含量,其相对含量占总挥发性组分峰面积的52.76%。结论:主要成分为:6,10,14-三甲基-2-十五烷酮(6.11%),1-辛烯-3-醇(5.74%),2,6-二(1,1-二甲基乙基)-4-甲基-苯酚(4.94%),双环戊二烯(4.47%),(E)-1,2,3-三甲基-4-丙烯基-萘(1.94%),正十五烷(1.88%),苯乙烯(1.76%),2,6,10,14-四甲基-十五烷(1.07%),正十六烷(1.06%),β-紫罗兰酮(1.05%)等化合物。Objective： The essential oil of trifolium repens was analyzed. Methods： The chemical constituents of the essential oil from flowers of Trifolium repens L. were analyzed by SPME - GC - MS. Results： The total essential oil were separated 76 components, the amount of the components from the essential oil were determined by peak area normalization method, the compon - ents separated were identified by GC - MS. Conclusion： There are 36 components which were identified, such as 2 - Pe - ntadecanone, 6, 10, 14 - trimethyl - （6.11%）, 1 - Octen - 3 - o1（5.74%）, Phenol, 2, 6 - bis（ 1,1 - dimethylethyl） - 4 - methyl - （4.94%）, Bicyclopentadiene（ 4.47% ）, Naphthalene, 1, 2, 3 - trimethyl - 4 - propenyl - （E） - （1.94%）, Pentadecane（1.88%）, Styrene（ 1. 76%）, Pentadecane, 2, 6, 10, 14 - tetramethyl - （ 1.07% ）, Hexadecane（ 1.06%）,. beta. - lonone（1.05%）, and these components are composed of about 52.76% of the total essential constituents.

关 键 词：固相微萃取 气相色谱/质谱 白车轴草 挥发性化学成分 

分 类 号：R284.1[医药卫生—中药学]