甘草提取物对酪氨酸酶活性的抑制作用  

Inhibitory effect of licoric extracts on activity of tyrosinase

作　　者：沈钧[1] 何莉萍[1] 张瑞斌[1] 

机构地区：[1]天津医科大学公共卫生学院卫生化学教研室,天津300070

出　　处：《中国临床药理学与治疗学》2006年第8期940-942,共3页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的研究甘草提取物对酪氨酸酶活性的抑制作用。方法用50%(V/V)乙醇提取甘草中的有效成分,分别以L-酪氨酸、邻苯二酚为底物,在475、390nm处测定溶液的吸光度,观察甘草提取物对单酚酶、双酚酶活性的影响。结果50%(V/V)乙醇甘草提取物对酪氨酸酶有明显的抑制作用(IC50分别为1.94、2.94mg·ml-1)。结论甘草提取物对酪氨酸酶单酚酶、双酚酶的活性均有抑制作用;以邻苯二酚为底物的双酚酶活性测定体系可较好地应用于酪氨酸酶活性的研究。AIM： To study the inhibitory effects of the licoric extracts on the activity of tyrosinase, which is constituted of monophenolase and diphenolase. METHODS： The 50%（V/V） alcohol is used as extractant. To investigate the effects of the lieoric extracts on the monophenolase and diphenolase activity, the absorbance of the solution containing several concentrations of the licoric extracts was measured at 475 nm and 390 nm, using the Ltyrosine and pyrocatechin as the substrate, respectively.RESULTS： The licoric extracts has showed a strongly inhibition on the activity of tyrosinase, either monophenolase or diphenolase. The IC50 is 1.94 and 2.94 mg·ml^-1 , respectively. CONCLUSION： The licoric extracts has inhibitory effects on both monophenolase and diphenolase. The diphenolase activity test system using pyrocatechin as the substrate is a useful procedure to study the activity of tyrosinase.

关 键 词：酪氨酸酶 甘草 VITC 黑色素 

分 类 号：R965.2[医药卫生—药理学]